Euphytica

, Volume 52, Issue 3, pp 183–191 | Cite as

Breaking the crossability barriers between disomic tetraploid Solanum acaule and tetrasomic tetraploid S. tuberosum

  • Masaru Iwanaga
  • Rosanna Freyre
  • Kazuo Watanabe
Article

Summary

A combination of compatible second pollinations and embryo rescue was applied for systematic production of true tetraploid hybrids from crosses between disomic tetraploid Solanum acaule and tetrasomic tetraploid potato, S. tuberosum. Several genotypes of tetraploid potatoes were pollinated with S. acaule, and the compatible second pollinations were made on the following day, with a genotype of S. phureja, IvP 35 to promote fruit development. Embryo rescue was carried out in 21 families, 14 to 27 days after the first pollination. A total of eight plants were obtained from the embryo rescue and their chromosome numbers were counted in the root tips. Three of the eight plants were identified as tetraploid, and five others as diploid. Morphology, isozyme banding patterns, and pollen stainability, as well as potato spindle tuber viroid (PSTVd) resistance, indicated the hybrid nature of the three plants. This is the first report of successful tetraploid hybrid production between disomic tetraploid S. acaule (4x) and tetrasomic tetraploid potatoes. Seed set from the crosses between one of hybrids and diploid potatoes indicated workable levels of both male and female fertility for introgression of valuable genes from S. acaule into the cultivated potato gene pool. The methodology used may be applied to other disomic tetraploid tuber-bearing Solanum species and with some modifications also to distantly related solanaceous species and genera.

Key words

tuber-bearing Solanum potato wild species germplasm enhancement counterfeit pollination disomic tetraploid embryo rescue interspecific hybridization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brown, C.R., 1988. Characterization of 2n pollen producing triploid hybrids between Solanum stoloniferum and cultivated diploid potatoes. Am. Potato J. 65: 75–84.Google Scholar
  2. Chase, S.S., 1963. Analytic breeding in Solanum tuberosum L.- A scheme utilizing parthenotes and other diploid stocks. Can. J. Genet. Cytol. 5: 359–363.Google Scholar
  3. Chavez, R., C.R. Brown & M. Iwanaga, 1988. Application of interspecific sesquiploidy to introgression of PLRV resistance from non-tuber-bearing Solanum etuberosum to cultivated potato germplasm. Theor. Appl. Genet. 76: 497–500.Google Scholar
  4. Chen, H.K., M.C. Mok, S. Shanmugasundaram & D.W.S. Mok, 1989. Interspecific hybridization between Vigna radiata (L.) Wilczek and V. glabrescens. Theor. Appl. Genet. 78: 641–647.Google Scholar
  5. Douches, D.S. & C.F. Quiros, 1988. Additional isozyme loci in tuber-bearing Solanums: inheritance and linkage relationships. J. Hered. 79: 377–384.Google Scholar
  6. Dvořák, J., 1983. Evidence for genetic suppression of heterogenetic chromosome pairing in polyploid species of Solanum, sect. Petota. Can. J. Genet. Cytol. 25: 530–539.Google Scholar
  7. Ehlenfeldt, M.K. & R.E. Hanneman Jr., 1988. Genetic control of Endosperm Balanced Number (EBN): three additive loci in a threshold-like system. Theor. Appl. Genet. 75: 825–832.Google Scholar
  8. Fish, N., A. Karp & M.G.K. Jones, 1988. Production of somatic hybrids by electrofusion in Solanum. Theor. Appl. Genet. 76: 260–266.Google Scholar
  9. Hadley, H.H. & S.J. Openshaw, 1980. Interspecific and intergeneric hybridization. In: W.R.Fehr & H.H.Hadley (Eds), Hybridization of Crop Plants. Amer. Soc. Agron., Madison, WI, USA, pp. 133–159.Google Scholar
  10. Hanneman, R.E. Jr. & J.B. Bamberg, 1986. Inventory of tuber-bearing Solanum species. Bulletin 533, College of Agriculture and Life Sciences, University of Wisconsin, Madison, Wisconsin, USA.Google Scholar
  11. Helgeson, J.P., G.T. Harberlach, J. Pohlman & S. Austin, 1988. Somatic fusions of Solanum species. Plant Cell Tissue Organ Culture 12: 185–187.Google Scholar
  12. Hermsen, J.G., Th. Ramanna & M.S. Ramanna, 1973. Double-bridging hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum. Euphytica 22: 557–566.Google Scholar
  13. Hermsen, J.G., Th. Verdenius & J. Verdenius, 1973. Selection from Solanum tuberosum group Phureja of genotypes combining high frequency haploid induction with homozygosity for embryospot. Euphytica 22: 244–259.Google Scholar
  14. Hermsen J.G., Th., 1984. Utilization of wide crosses in potato breeding. In: Present and Future Strategies for Potato Breeding and Improvement. Rept. XXVI Plan. Conf., CIP, Lima, Peru, pp. 115–132.Google Scholar
  15. International Potato Center, 1990. Annual Report. International Potato Center, Apartado 5969, Lima, Peru.Google Scholar
  16. Iwanaga, M., 1984. Discovery of a synaptic mutant in potato haploids and its usefulness for potato breeding. Theor. Appl. Genet. 68: 87–93.Google Scholar
  17. Iwanaga, M., & S.J. Peloquin, 1982. Origin and evolution of cultivated tetraploid potatoes via 2n gametes. Theor. Appl. Genet. 61: 161–169.Google Scholar
  18. Iwanaga, M., P. Jatala, R. Ortiz & E. Guevara, 1989. Use of FDR 2n pollen to transfer resistance to root-knot nematodes into cultivated 4x potatoes. J. Amer. Soc. Hort. Sci. 114: 1008–1013.Google Scholar
  19. Johnston, S.A., T.P.M. den Nijs, S.J. Peloquin, R.E. Hanneman Jr., 1980. The significance of genic balance to endosperm development in interspecific crosses. Theor. Appl. Genet. 57: 5–9.Google Scholar
  20. Lamm, R., 1953. Investigations of some tuber-bearing Solanum hybrids. Hereditas 39: 97–112.Google Scholar
  21. Marks, G.E., 1954. An aceto-carmine glycerol jelly for use in pollen fertility counts. Stain Tech. 29: 277.Google Scholar
  22. Peloquin, S.J., 1983. Genetic engineering with meiotic mutants. In: D.L.Mulcahy & E.Ottaviano (Eds), Pollen: Biology and Implication for Plant Breeding. Elsevier, NY, pp. 151–155.Google Scholar
  23. Propach, H., 1938. Kreuzbarkeit von Solanum-Arten untereinander und mit Kulturkartoffeln und die Fertilität der ßastarde. Forschungsdienst. 6: 311–314.Google Scholar
  24. Ramanna, M.S. & J.G. Hermsen, Th., 1971. Somatic chromosome elimination and meiotic chromosome pairing in the triple hybrid 6x-(Solanum acaule × S. bulbocastanum) × 2x-S. phureja. Euphytica 20: 470–481.Google Scholar
  25. Ross, H., 1986. Potato Breeding-Problems and Perspectives. Verlag Paul Parey, Berlin and Hamburg.Google Scholar
  26. Schmiediche, P.E., J.G. Hawkes & C.M. Ochoa, 1982. The breeding of the cultivated potato species Solanum × juzepczukii and S. × curtilobum. II. The resynthesis of S. × juzepczukii and S. × curtilobum. Euphytica 31: 695–707.Google Scholar
  27. Spack, V., 1985. Cultivo de ovários, óvulos y embryones de papa. Thesis Msc, Universidad Nacional Agraria, Lima, Peru.Google Scholar
  28. Swaminathan, M.S., 1954. Nature of polyploidy in some 48 chromosomes species of the genus Solanum section Tuberarium. Genetics 39: 59–76.Google Scholar
  29. Watanabe, K., 1988. Occurrence, Frequency, Cytology and Genetics of 2n Pollen; and Sexual Polyploidization in tuber-bearing Solanum. Ph.D. Thesis, University of Wisconsin, Madison, Wisconsin, USA.Google Scholar
  30. Watanabe, K., C. Arbizu, P.E. Schmiediche & M.T. Jackson, 1990. Germplasm enhancement methods for disomic tetraploid species of Solanum with special references to S. acaule. Am. Potato J. 67: 586.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Masaru Iwanaga
    • 1
  • Rosanna Freyre
    • 1
  • Kazuo Watanabe
    • 1
  1. 1.International Potato Center (CIP)LimaPeru

Personalised recommendations