Plant Molecular Biology

, Volume 23, Issue 2, pp 365–376 | Cite as

Cloning and characterization of the lectin cDNA clones from onion, shallot and leek

  • Els J. M. Van Damme
  • Koen Smeets
  • Iris Engelborghs
  • Helen Aelbers
  • Jan Balzarini
  • Arpad Pusztai
  • Fred van Leuven
  • Irwin J. Goldstein
  • Willy J. Peumans
Research Articles

Abstract

Characterization of the lectins from onion (Allium cepa), shallot (A. ascalonicum) and leek (A. porrum) has shown that these lectins differ from previously isolated Alliaceae lectins not only in their molecular structure but also in their ability to inhibit retrovirus infection of target cells.

cDNA libraries constructed from poly(A)-rich RNA isolated from young shoots of onion, shallot and leek were screened for lectin cDNA clones using colony hybridization. Sequence analysis of the lectin cDNA clones from these three species revealed a high degree of sequence similarity both at the nucleotide and at the amino acid level.

Apparently the onion, shallot and leek lectins are translated from mRNAs of ca. 800 nucleotides. The primary translation products are preproproteins (ca. 19 kDa) which are converted into the mature lectin polypeptides (12.5–13 kDa) after post-translational modifications.

Southern blot analysis of genomic DNA has shown that the lectins are most probably encoded by a family of closely related genes which is in good agreement with the sequence heterogeneity found between different lectin cDNA clones of one species.

Key words

Allium cDNA cloning lectin retrovirus inhibition 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Els J. M. Van Damme
    • 1
  • Koen Smeets
    • 1
  • Iris Engelborghs
    • 1
  • Helen Aelbers
    • 1
  • Jan Balzarini
    • 2
  • Arpad Pusztai
    • 3
  • Fred van Leuven
    • 4
  • Irwin J. Goldstein
    • 5
  • Willy J. Peumans
    • 1
  1. 1.Laboratory for Phytopathology and Plant ProtectionKatholieke Universiteit LeuvenLeuven
  2. 2.Laboratory of Experimental Chemotherapy, Rega Institute for Medical ResearchKatholieke Universiteit LeuvenBelgium
  3. 3.The Rowette Research InstituteAberdeenUK
  4. 4.Center for Human GeneticsKatholieke Universiteit LeuvenBelgium
  5. 5.Department of Biological ChemistryUniversity of MichiganAnn ArborUSA

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