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Plant Molecular Biology

, Volume 23, Issue 2, pp 279–286 | Cite as

A view of plant dehydrins using antibodies specific to the carboxy terminal peptide

  • Timothy J. Close
  • Raymond D. Fenton
  • Francis Moonan
Research Articles

Abstract

Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. This range covered two families of monocots, viz. Gramineae (Hordeum vulgare L., Triticum aestivum L., Zea mays L., Oryza sativa L.) and Liliaceae (Allium sativa L.), and five families of dicots, Malvaceae (Gossypium hirsutum L.), Solanaceae (Lycopersicon esculentum L.), Brassicaceae (Raphanus sativus L.), Fabaceae (Vigna unguiculata L.), and Cucurbitaceae (Cucumis sativus L.). Two families of gymnosperms, Pinaceae (Pinus edulis Engelm.) and Ginkgoaceae (Ginkgo biloba L.), were also included. For several plants in which dehydrin cDNA and genomic clones have previously been characterized, it now appears that the dehydrin family of proteins is larger, and the regulation of dehydrin expression much more complex, than earlier studies have shown.

Key words

dehydrin LEA D-11 stress protein 

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References

  1. 1.
    Close TJ, Kortt AA, Chandler PM: A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108 (1989).PubMedGoogle Scholar
  2. 2.
    Close TJ, Chandler PM: Cereal dehydrins: serology, gene mapping and potential functional roles. Aust J Plant Physiol 17: 333–344 (1990).Google Scholar
  3. 3.
    Close TJ, Fenton RD, Yang A, Asghar R, DeMason DA, Crone DE, Meyer NC, Moonan F. Dchydrin: the protein. In: Close TJ, Bray EA,(eds) Response of Plants to Cellular Dehydration During Environmental Stress, pp. 104–114. American Society of Plant Physiologists (1993).Google Scholar
  4. 4.
    Cohen A, Plant AL, Moses MS, Bray EA. Organ-specific and environmentally-regulated expression of two ABA-induced genes of tomato. Nucleotide sequence and analysis of the corresponding cDNAs. Plant Physiol 97: 1367–1384 (1991).Google Scholar
  5. 5.
    Dure LIII, Crouch M, Harada J, Ho T-HD, Mundy J, Quatrano R, Thomas T, Sung ZR: Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).Google Scholar
  6. 6.
    Dure L III: Structural motifs in LEA proteins. In: Close TJ, Bray EA (eds) Response of Plants to Cellular Dehydration During Environmental Stress, pp. 91–103. American Society of Plant Physiologists (1993).Google Scholar
  7. 7.
    Galau GA, Close TJ: Sequences of the cotton Group 2/LEA/RAB/dehydrin proteins encoded by Lea3 cDNAs. Plant Physiol 98: 1523–1525 (1992).Google Scholar
  8. 8.
    Godoy JA, Pardo JM: Pintortoro JA: A tomato cDNA inducible by salt stress and abscisic acid-nucleotide sequence and expression pattern. Plant Mol Biol 15: 695–705 (1990).CrossRefPubMedGoogle Scholar
  9. 9.
    Gulick PJ, Dvorak J: Coordinate gene response to salt stress in Lophopyrum elongatum. Plant Physiol 100: 1384–1388 (1992).Google Scholar
  10. 10.
    Guo W, Ward RW, Thomashow MF: Characterization of a cold-regulated wheat gene related to Arabidopsis Cor47. Plant Physiol 100: 915–922 (1992).Google Scholar
  11. 11.
    Guy C, Haskell D, Neven L, Klein P, Smelser C: Hydration-state-responsive proteins link cold and drought stress in spinach. Planta 188: 265–270 (1992).CrossRefGoogle Scholar
  12. 12.
    Harlow E, Lane D. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1988).Google Scholar
  13. 13.
    Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD: Construction of a restriction fragment length polymorphims map for barley. Genome 34: 438–447 (1991).Google Scholar
  14. 14.
    Houde M, Danyluk J, Laliberte J-F, Rassart E, Dhindsa RS, Sarhan F: Cloning, characterization and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. Plant Physiol 99: 1381–1387 (1992).Google Scholar
  15. 15.
    Hughes DW, Galau GA: Temporally modular gene expression during cotyledon development. Genes Devel 3: 358–369 (1989).PubMedGoogle Scholar
  16. 16.
    Jacobsen JV, Shaw DC: Heat-stable proteins and abscisic acid action in barley aleurone cells. Plant Physiol 91: 1520–1526 (1989).Google Scholar
  17. 17.
    King SW, Joshi CP, Nguyen HT: DNA sequence of an ABA-responsive gene (rab 15) from water-stressed wheat roots. Plant Mol Biol 18: 119–121 (1992).CrossRefPubMedGoogle Scholar
  18. 18.
    Kleinhofs A, Kilian A, Maroof MAS, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ: A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet, in press (1993).Google Scholar
  19. 19.
    Kusano T, Aguan K, Abe M, Sugawara K: Nucleotide sequence of a rice rab16 homologue gene. Plant Mol Biol 18: 127–129 (1992).CrossRefPubMedGoogle Scholar
  20. 20.
    Morris CF, Anderberg RJ, Goldmark PJ, Walker-Simmons MK: Molecular cloning and expression of abscisic acid responsive genes in embryos of dormant wheat seeds. Plant Physiol 95: 814–821 (1991).Google Scholar
  21. 21.
    Plana M, Itarte E, Eritja R, Goday A, Pages M, Martinez MC: Phosphorylation of maize RAB-17 protein by casein kinase-2. J Biol Chem 266: 22510–22514 (1991).PubMedGoogle Scholar
  22. 22.
    Raynal M, Gaubier P, Grellet F, Delseny M: Nucleotide sequence of a radish cDNA clone coding for a late embryogenesis abundant (LEA) protein. Nucl Acids Res 18: 6132 (1990).PubMedGoogle Scholar
  23. 23.
    Reid JL, Walker-Simmons MK: Synthesis of abscisic acid-responsive, heat stable proteins in embryonic axes of dormant wheat grains. Plant Physiol 93: 662–667 (1990).Google Scholar
  24. 24.
    Skriver K, Mundy J: Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2: 503–512 (1990).CrossRefPubMedGoogle Scholar
  25. 25.
    Vilardell J, Goday A, Freire MA, Torrent M, Martinez MC, Torne JM, Pages M: Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize. Plant Mol Biol 14: 423–432 (1990).PubMedGoogle Scholar
  26. 26.
    Yamaguchi-Shinozaki K, Mundy J, Chua N-H: Four tightly linked rab genes are differentially expressed in rice. Plant Mol Biol 14: 29–39 (1989).CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Timothy J. Close
    • 1
  • Raymond D. Fenton
    • 1
  • Francis Moonan
    • 1
  1. 1.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA

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