Plant Molecular Biology

, Volume 20, Issue 6, pp 1195–1197

New plant binary vectors with selectable markers located proximal to the left T-DNA border

  • Detlef Becker
  • Elke Kemper
  • Jeff Schell
  • Robert Masterson
Update Section Short Communication

Abstract

Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and β-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.

Key words

Agrobacterium tumefaciens T-DNA plant transformation binary vectors selectable markers 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Detlef Becker
    • 1
  • Elke Kemper
    • 1
  • Jeff Schell
    • 1
  • Robert Masterson
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKöln 30Germany

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