Characterisation of the cDNA clones of two β-tubulin genes and their expression in the potato plant (Solanum tuberosum L.)
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The cDNA clones of two potato β-tubulin genes were isolated from a tuberising stolon tip library. Analysis of 20 positive clones showed that they represented one or another of two different but very similar β-tubulin genes, designated TUBST1 and TUBST2. The expression pattern of β-tubulin genes in the potato plant was investigated by RNA blot analysis and by RT-PCR. Southern analysis of potato genomic DNA with coding and non-coding β-tubulin probes revealed that there are multiple β-tubulin genes in the potato genome and that there is likely to be considerable divergence in the 3′ non-coding sequences. Phylogenetic analysis of plant β-tubulin genes is described.
Key wordsreverse transcriptase PCR (RT-PCR) Solanum tuberosum tuberisation β-tubulin
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