Plant Molecular Biology

, Volume 26, Issue 3, pp 1013–1018 | Cite as

Characterisation of the cDNA clones of two β-tubulin genes and their expression in the potato plant (Solanum tuberosum L.)

  • Mark A. Taylor
  • Frank Wright
  • Howard V. Davies
Short Communication


The cDNA clones of two potato β-tubulin genes were isolated from a tuberising stolon tip library. Analysis of 20 positive clones showed that they represented one or another of two different but very similar β-tubulin genes, designated TUBST1 and TUBST2. The expression pattern of β-tubulin genes in the potato plant was investigated by RNA blot analysis and by RT-PCR. Southern analysis of potato genomic DNA with coding and non-coding β-tubulin probes revealed that there are multiple β-tubulin genes in the potato genome and that there is likely to be considerable divergence in the 3′ non-coding sequences. Phylogenetic analysis of plant β-tubulin genes is described.

Key words

reverse transcriptase PCR (RT-PCR) Solanum tuberosum tuberisation β-tubulin 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cleveland, DW, Sullivan, KF: Molecular biology and genetics of tubulin. Annu Rev Biochem 54: 331–365 (1985).CrossRefPubMedGoogle Scholar
  2. 2.
    Draper, J, Scott, RJ, Armitage, P, Walden, R: Plant Genetic Transformation and Gene Expression: A Laboratory Manual. Blackwell Scientific Publications, London (1988).Google Scholar
  3. 3.
    Duckett, CM, Lloyd, CW: Gibberellic acid-induced microtubule reorientation in dwarf peas is accompanied by rapid modification of an α-tubulin isotype. Plant J 5: 363–372 (1994).Google Scholar
  4. 4.
    Feinberg, AP, Vogelstein, B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266 (1984).PubMedGoogle Scholar
  5. 5.
    Guiltinan, MJ, Ma, DP: Barker, RF, Bustos, MM, Cyr, RJ, Yadegari, R, Fosket, DE: The isolation, characterisation and sequence of two divergent β-tubulin genes from soybean (Glycine max L.). Plant Mol Biol 10: 171–184 (1987).Google Scholar
  6. 6.
    Gunning, BES, Hardham, AR: Microtubules. Annu Rev Plant Physiol 33: 651–698 (1982).CrossRefGoogle Scholar
  7. 7.
    Hedges, SB: The number of replications needed for accurate estimation of the bootstrap P value in phylogenetic studies. Mol Biol Evol 9: 366–369 (1992).PubMedGoogle Scholar
  8. 8.
    Hedley, PE, Machray, GC, Davies, HV, Burch, L, Waugh, R: cDNA cloning and expression of a potato (Solanum tuberosum) invertase. Plant Mol Biol 22: 917–922 (1993).CrossRefPubMedGoogle Scholar
  9. 9.
    Hussey, PJ, Gull, K: Multiple isotypes of α- and β-tubulin in the plant Phaeolus vulgaris. FEBS Lett 181: 113–118 (1985).CrossRefGoogle Scholar
  10. 10.
    Hussey, PJ, Lloyd, CW, Gull, K: Differential and developmental expression of β-tubulins in a higher plant. J Biol Chem 263: 5474–5479 (1988).PubMedGoogle Scholar
  11. 11.
    Hussey, PJ, Haas, N, Hunsperger, J, Larkin, J, Snustad, DP, Silflow, CD: The β-tubulin gene family in Zea mays: two differentially expressed β-tubulin genes. Plant Mol Biol 15: 957–972 (1990).PubMedGoogle Scholar
  12. 12.
    Hussey, PJ, Snustad, DP, Silflow, CD: Tubulin gene expression in higher plants. In: Lloyd, CW (ed) The Cytoskeletal Basis of Plant Growth and Form, pp. 15–27. Academic Press, London (1991).Google Scholar
  13. 13.
    Joshi, CH, Cleveland, DW: Diversity among tubulin subunits: towards what functional end? Cell Motil Cytoskeleton 16: 159–163 (1990).PubMedGoogle Scholar
  14. 14.
    Kimura, M: The neutral theory of molecular evolution. Cambridge University Press, Cambridge, UK (1983).Google Scholar
  15. 15.
    Kopczak, SD, Haas, NA, Hussey, PJ, Silflow, CD, Snustad, DP: The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell 4: 539–547 (1992).CrossRefPubMedGoogle Scholar
  16. 16.
    Liaud, MF, Brinkmann, H, Cerff, R: The β-tubulin gene family of pea: Primary structure, genomic organisation and intron-dependent evolution of genes. Plant Mol Biol 18: 639–651 (1992).Google Scholar
  17. 17.
    Lloyd, CW: The plant cytoskeleton: the influence of fluorescence microscopy. Annu Rev Plant Physiol 38: 119–139 (1987).Google Scholar
  18. 18.
    Raff, EC: Genetics of microtubule systems. J Cell Biol 98: 1–10 (1984).CrossRefPubMedGoogle Scholar
  19. 19.
    Saitou, N, Nei, M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 (1987).PubMedGoogle Scholar
  20. 20.
    Simpson, CG, Sinibaldi, R, Brown, JWS: Rapid analysis of plant gene expression by a novel reverse transcriptase-PCR method. Plant J 2: 835–836 (1992).Google Scholar
  21. 21.
    Snustad, PD, Haas, NA, Kopczak, SD, Silflow, CD: The small genome of Arabidopsis contains at least nine expressed β-tubulin genes. Plant Cell 4: 549–556 (1992).CrossRefPubMedGoogle Scholar
  22. 22.
    Taylor, MA, Davies, HV, Scobie, LA: Molecular changes associated with tuberisation in Solanum tuberosum. Differential expression of α-tubulin isotypes. Physiol Plant 81: 244–250 (1991).CrossRefGoogle Scholar
  23. 23.
    Taylor, MA, Mad, Arif SA, Kumar, A, Davies, HV, Scobie, LA, Pearce, SR, Flavell, AJ: Expression and sequence analysis of cDNAs induced during the early stages of tuberisation in different organs of the potato plant (S. tuberosum L.). Plant Mol Biol 20: 641–651 (1992).PubMedGoogle Scholar
  24. 24.
    Taylor, MA, Mad, Arif SA, Pearce, SR, Davies, HV, Kumar, A, George, LA: Differential expression and sequence analysis of ribosomal protein genes induced in stolon tips of potato (Solanum tuberosum L.) during the early stages of tuberization. Plant Physiol 100: 1171–1176 (1992).Google Scholar
  25. 25.
    Villemur, R, Haas, NA, Joyce, CM, Snustad, DP, Silflow, CD: Characterisation of four new β-tubulin genes and their expression during male flower development in maize (Zea mays L.). Plant Mol Biol 24: 295–315 (1994).PubMedGoogle Scholar
  26. 26.
    Vreugdenhil, D, Struik, PC: An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum). Physiol Plant 75: 525–531 (1989).Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Mark A. Taylor
    • 1
  • Frank Wright
    • 2
  • Howard V. Davies
    • 1
  1. 1.Department of Cellular and Environmental PhysiologyScottish Crop Research InstituteDundeeUK
  2. 2.Scottish Agricultural Statistics ServiceUniversity of EdinburghEdinburghUK

Personalised recommendations