Photosynthesis Research

, Volume 16, Issue 3, pp 277–284 | Cite as

Rapid, nondestructive measurement of chlorophyll content in leaves with nonuniform chlorophyll distribution

  • L. A. Spomer
  • M. A. L. Smith
  • J. S. Sawwan
Technical Communication


A practical microcomputerized video image analysis method is described for quantifying leaf chlorophyll content without extraction. Chlorophyll concentration is estimated from densimetric measurements of whole, intact leaves. Direct comparison with conventional extraction measurements on Epipremnum aureum, a variegated species, verified the image analysis technique's accuracy. The inherent advantages with regard to the nondestructive and convenient nature of the measurement, and suitability for leaves with irregular chlorophyll distribution, are discussed.

Key words

chlorophyll concentration chlorophyll measurement densitometry Epipremnum microcomputerized image analysis 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • L. A. Spomer
    • 1
  • M. A. L. Smith
    • 1
  • J. S. Sawwan
    • 1
  1. 1.Department of HorticultureUniversity of IllinoisUrbanaUSA

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