Plant Molecular Biology

, Volume 14, Issue 3, pp 433–443 | Cite as

Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter

  • Margaret Sanger
  • Steve Daubert
  • Robert M. Goodman
Article

Abstract

A segment of DNA from the genome of figwort mosaic virus (FMV) strain M3 possesses promoter activity when tested in electroporated protoplasts from, and transgenic plants of, Nicotiana tabacum cv. Xanthi nc. The 1.1 kb DNA segment, designated the ‘34S’ promoter, is derived from a position on the FMV genome comparable to the position on the cauliflower mosaic virus (CaMV) genome containing the 35S promoter. The 34S and 35S promoters show approximately 63% nucleotide homology in the TATA, CCACT, and −18 to +1 domains, but in sequences further upstream the homology drops below 50%. Promoter activities were estimated using β-glucuronidase and neomycin phosphotransferase II reporter gene systems. The activity of the 34S promoter segment approximates that of the 35S promoter in both protoplast transient expression assays and in stably transformed tobacco plants. Truncation of 5′ sequences from the 34S promoter indicates that promoter strength depends upon DNA sequences located several hundred nucleotides upstream from the TATA box. In leaf tissue the 34S promoter is 20-fold more active than the mannopine synthase (MAS) promoter from Agrobacterium tumefaciens T-DNA. The 34S promoter lacks the root-specific and wound-stimulated expression of the MAS promoter, showing relatively uniform root, stem, leaf, and floral activities.

Key words

cauliflower mosaic 35S figwort mosaic mannopine synthase inducibility plant promoters 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    An G: Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells. Plant Physiol 81: 86–91 (1986).Google Scholar
  2. 2.
    Ausbel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds): Current Protocols in Molecular Biology. John Wiley and Sons, New York (1987).Google Scholar
  3. 3.
    Balazs E, Guilley H, Jonard G, Richards K: Nucleotide sequence of DNA from an altered-virulence isolate D/H of the cauliflower mosaic virus. Gene 19: 239–249 (1982).CrossRefPubMedGoogle Scholar
  4. 4.
    Barker RF, Idler KB, Thompson DV, Demp JD: Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi 15955. Plant Mol Biol 2: 335–350 (1983).Google Scholar
  5. 5.
    Benfey PN, Ren L, Chua N-H: The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J 8: 2195–2202 (1989).Google Scholar
  6. 6.
    Bio-Rad: Automated protein assay. Bulletin 1177 (1984).Google Scholar
  7. 7.
    Bonneville J, Hohn T, Pfeiffer P: Reverse transcription in the plant virus, cauliflower mosaic virus. In: DomingoE, HollandJ, Ahlquist P (eds) RNA Genetics, vol 2, pp. 23–42. CRC Press, Boca Raton, FL (1988).Google Scholar
  8. 8.
    Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 (1976).CrossRefPubMedGoogle Scholar
  9. 9.
    Burzio LO, Brito M, Zarraga AM, Siddiqui MAQ: Assay of chloramphenicol acetyltransferase by high-performance liquid chromatography. Gene Anal Techn 5: 5–8 (1988).Google Scholar
  10. 10.
    Comai L, Facciotti D, Hiatt WR, Thompson G, Rose E, Stalker DM: Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744 (1985).Google Scholar
  11. 11.
    Dean C, Favreau M, Tamaki S, Bond-Nutter D, Dunsmuir P, Bedbrook J: Expression of tandem gene fusions in transgenic tobacco plants. Nucl Acids Res 15: 7601–7617 (1988).Google Scholar
  12. 12.
    Fang R-X, Nagy F, Sivasubramaniam S, Chua N-H: Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants. Plant Cell 1: 141–150 (1989).CrossRefPubMedGoogle Scholar
  13. 13.
    Fang R, Wu X, Bu M, Tian Y, Cai F, Mang K: Complete nucleotide sequence of cauliflower mosaic virus (Xinjing isolate) genomic DNA. Chinese J Virol 1: 247–256 (1985).Google Scholar
  14. 14.
    Franck A, Guilley H, Jonard G, Richards K, Hirth L: Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21: 285–294 (1980).CrossRefPubMedGoogle Scholar
  15. 15.
    Fromm M, Taylor LP, Wolbot V: Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82: 5824–5828 (1985).PubMedGoogle Scholar
  16. 16.
    Fromm ME, Taylor LP, Wolbot V: Stable transformation of maize after gene transfer by electroporation. Nature 319: 791–793 (1986).PubMedGoogle Scholar
  17. 17.
    Gardner RC, Howarth AJ, Hahn P, Brown-Leudi M, Shepherd RJ: The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucl Acids Res 9: 2871–2881 (1981).PubMedGoogle Scholar
  18. 18.
    Gorman CM, Moffat LF, Howard BH: Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. Mol Cel Biol 2: 1044–1051 (1982).Google Scholar
  19. 19.
    Guilley H, Dudley RK, Jonard G, Balàzs E, Richards KE: Transcription of cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts. Cell 30: 763–773 (1982).CrossRefPubMedGoogle Scholar
  20. 20.
    Hoekema A, Hirsch PR, Hooykaas PJ, Schilperoort RA: A binary plant vector strategy based on separation of vir-and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180 (1983).Google Scholar
  21. 21.
    Hoekema A, Huisman MJ, Molendijk L, van den Elzen PJ, Cornelissen BJ: The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/Technology 7: 273–278 (1989).Google Scholar
  22. 22.
    Holstus MD, deWarle D, Depicker A, Messens E, VanMontagu M, Schell J: Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163: 181–187 (1979).Google Scholar
  23. 23.
    Hull R, Sadler J, Longstaff M: The sequence of carnation etched ring viral. DNA: comparison with cauliflower mosaic virus and retroviruses. EMBO J 5: 3083–3090 (1986).Google Scholar
  24. 24.
    Jefferson RA: Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5(4): 387–405 (1987).Google Scholar
  25. 25.
    Jefferson RA, Kavanagh TA, Bevan MV: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).PubMedGoogle Scholar
  26. 26.
    Jones JD, Gilbert DE, Grady KL, Jorgensen RA: T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207: 478–485 (1987).CrossRefGoogle Scholar
  27. 27.
    Langridge WHR, Fitzgerald KJ, Koncz C, Schell J, Szaly AA: Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones. Proc Natl Acad Sci USA 86: 3219–3232 (1989).Google Scholar
  28. 28.
    Lin W, Odell JT, Schreiner RM: Soybean protoplast culture and direct gene uptake and expression by cultured soybean protoplasts. Plant Physiol 84: 856–861 (1987).Google Scholar
  29. 29.
    Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).Google Scholar
  30. 30.
    McDonnell RE, Clark RD, Smith WA, Hinchee MA: A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues. Plant Mol Biol Rep 5: 380–386 (1987).Google Scholar
  31. 31.
    Murashige T, Skoog F: A revised medium for rapid growth and bio-assay with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).Google Scholar
  32. 32.
    Nagy F, Odell JT, Morelli G, Chua N-H: Properties of expression of the 35S promoter from CaMV in transgenic tobacco plants. In: Zaitlin M, Day P, Hollaender A (eds) Biotechnology in Plant Science: Relevance to Agriculture in the Eighties, pp. 227–236. Academic Press, New York (1985).Google Scholar
  33. 33.
    Odell JT, Knowlton S, Lin W, Mauvais J: Properties of an isolated transcription stimulating sequence derived from the cauliflower mosaic virus 35S promoter. Plant Mol Biol 10: 263–272 (1988).Google Scholar
  34. 34.
    Odell JT, Nagy F, Chua N-H: Identification of sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810–812 (1985).PubMedGoogle Scholar
  35. 35.
    Odell JT, Nagy F, Chua N-H: Variability in 35S promoter expression between independent transformants. In: Key J, McIntosh L (eds), Plant Gene Systems and Their Biology, vol 62, pp. 321–329. Alan R. Liss, New York (1987).Google Scholar
  36. 36.
    Ou-Lee TM, Turgeon R, Wu R: Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice wheat, and sorghum. Proc Natl Acad Sci USA 83: 6815–6819 (1986).Google Scholar
  37. 37.
    Ow DW, Jacobs JD, Howell SH: Functional regions of the cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proc Natl Acad Sci USA 84: 4870–4874 (1987).Google Scholar
  38. 38.
    Ow DW, Wood KV, De Luca M, de Wet JR, Helinski DR, Howell SH: Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856–859 (1986).Google Scholar
  39. 39.
    Richins RD, Scholthof HB, Shepherd RJ: Sequence of figwort mosaic virus DNA (caulimovirus group). Nucl Acids Res 15: 8451–8466 (1987).PubMedGoogle Scholar
  40. 40.
    Sanders PR, Winters JA, Barnason AR, Rogers SG, Fraley RT: Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucl Acids Res 15: 1543–1558 (1987).PubMedGoogle Scholar
  41. 41.
    Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1974).Google Scholar
  42. 42.
    Shepherd RJ, Richins RD, Duffus JE, Handley MK: Figwort mosaic virus: properties of the virus and its adaption to a new host. Phytopathology 77: 1668–1673 (1987).Google Scholar
  43. 43.
    Stratfor dR, Plaskitt K, Turner D, Markham P, Covey S: Molecular properties of Bari 1, a mild strain of cauliflower mosaic virus. J Gen Virol 69: 2375–2386 (1988).Google Scholar
  44. 44.
    Teere TH, Lehväslaiho H, Franck M, Uotila J, Heino P, Palva ET, VanMontagu M, Herrera-Estrella L: Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants. EMBO J 8: 343–350 (1989).PubMedGoogle Scholar
  45. 45.
    Winter JA, Wright RL, Gurley WB: Map locations of five transcripts homologous to TR-DNA in tobacco and sunflower crown gall tumors. Nucl Acids Res 12: 2391–2406 (1984).PubMedGoogle Scholar
  46. 46.
    Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103–119 (1985).CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Margaret Sanger
    • 1
    • 2
  • Steve Daubert
    • 2
  • Robert M. Goodman
    • 1
  1. 1.Calgene Inc.DavisUSA
  2. 2.Department of Plant PathologyUniversity of CaliforniaDavis

Personalised recommendations