Advertisement

Plant Molecular Biology

, Volume 17, Issue 6, pp 1139–1154 | Cite as

A detailed study of the regulation and evolution of the two classes of patatin genes in Solanum tuberosum L.

  • Xiang-Yun Liu
  • Mario Rocha-Sosa
  • Sabine Hummel
  • Lothar Willmitzer
  • Wolf B. Frommer
Article

Abstract

The class-specific expression of patatin genes was investigated by analysing four new patatin genes. A class I patatin gene from cv. Berolina as well as a class I and two class II patatin genes from the monohaploid cultivar AM 80/5793 were isolated and partially sequenced. Sequence comparison indicates rearrangements as the major source for the generation of diversity between the different members of the classes. The expression of single genes was studied in potato plants transformed with chimaeric genes where the putative patatin promoters were fused to the GUS reporter gene. A detailed histochemical analysis reveals that both class I genes are expressed as the previously described class I patatin gene B33 from cv. Berolina [1], i.e. in the starch-containing cells of potato tubers and in sucrose-induced leaves. The class II gene pgT12 shows the same pattern as the previously described class II gene pgT2 [2], i.e. expression in root tips and in the vascular tissue of tubers, whereas no activity was detectable for pgT4. Thus the expression pattern of both classes of genes seems to be stable at least within or even between different cultivars.

Key words

patatin promoter β-glucuronidase potato 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Rocha-Sosa M, Sonnewald U, Frommer WB, Stratmann M, Schell J, Willmitzer L: Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 8: 23–29 (1989).Google Scholar
  2. 2.
    Köster-Töpfer M, Frommer WB, Rocha-Sosa M, Rosahl S, Schell J, Willmitzer L: A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants. Mol Gen Genet 219: 390–396 (1989).Google Scholar
  3. 3.
    Cutter EG: Structure and development of the potato plant. In: Harris PM (ed) The Potato Crop: The Scientific Basis for Improvement, pp. 70–152. Chapman & Hall, London (1987).Google Scholar
  4. 4.
    Racusen D, Foote M: A major soluble glycoprotein of potato tubers. J Food Biochem 4: 43–52 (1980).Google Scholar
  5. 5.
    Dennis S, Galliard T: Wax ester formation catalysed by isoenzymes of lipolytic acyl hydrolase. Phytochemistry 13: 2469–2473 (1974).Google Scholar
  6. 6.
    Racusen D: Patatin and esterase in Désirée potato tuber. J Food Ind 9: 361–367 (1985).Google Scholar
  7. 7.
    Twell D, Ooms G: Structural diversity of the patatin gene family in potato cv. Désirée. Mol Gen Genet 212: 325–336 (1988).Google Scholar
  8. 8.
    Park WD, Blackwood C, Mignery GA, Hermodson MA, Lister RM: Analysis of the heterogeneity of the 40.000 molecular weight tuber glycoprotein of potatoes by immunological methods and by N-terminal sequence analysis. Plant Physiol 71: 156–160 (1983).Google Scholar
  9. 9.
    Mignery GA, Pikaard CS, Hannapel DJ, Park WD: Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucl Acids Res 12: 7987–8000 (1984).Google Scholar
  10. 10.
    Stiekema WJ, Heidekamp F, Dirkse WG, Beckum JV, Haan PD, Bosch CT, Louwerse JD: Molecular cloning and analysis of four potato tuber mRNAs. Plant Mol Biol 11: 255–269 (1988).Google Scholar
  11. 11.
    Nakamura K, Hattori T, Asahi T: Direct immunological identification of full length cDNA clones for plant protein without gene fusion to E. coli protein. FEBS Lett 198: 16–20 (1986).Google Scholar
  12. 12.
    Bevan M, Barker R, Goldsbrough A, Jarvis M, Kavanagh T, Iturriaga G: The structure and transcription start site of a major potato tuber protein gene. Nucl Acids Res 14: 4625–4638 (1986).Google Scholar
  13. 13.
    Mignery GA, Pikaard CS, Park WD: Molecular characterisation of the patatin multigene family of potato. Gene 62: 27–44 (1988).Google Scholar
  14. 14.
    Rosahl S, Schmidt R, Schell J, Willmitzer L: Isolation and characterisation of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers. Mol Gen Genet 203: 214–220 (1986).Google Scholar
  15. 15.
    Wenzler H, Mignery G, Fisher L, Park W: Analysis of a chimeric class I patatin-GUS gene in transgenic potato plants. Plant Mol Biol 12: 41–50 (1989).Google Scholar
  16. 16.
    Jefferson R, Goldsbrough A, Bevan M: Transcriptional regulation of a patatin-1 gene in potato. Plant Mol Biol 14: 995–1006 (1990).Google Scholar
  17. 17.
    Wenzler H, Mignery G, Fisher L, Park W: Sucrose-regulated expression of a chimeric potato tuber gene in leaves of transgenic tobacco plants. Plant Mol Biol 13: 347–354 (1989).Google Scholar
  18. 18.
    Rogers SO, Bendich AJ: Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5: 69–76 (1985).Google Scholar
  19. 19.
    Rosahl S, Eckes P, Schell J, Willmitzer L: Organ-specific gene expression in potato: isolation and characterisation of tuber-specific cDNA sequences. Mol Gen Genet 202: 368–373 (1986).Google Scholar
  20. 20.
    Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).Google Scholar
  21. 21.
    Jefferson RA: Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).Google Scholar
  22. 22.
    Köster-Töpfer M, Frommer WB, Rocha-Sosa M, Willmitzer L: Presence of a transposon-like element in the promoter region of an inactive patatin gene in Solanum tuberosum L. Plant Mol Biol 14: 239–247 (1990).Google Scholar
  23. 23.
    Liu XJ, Prat S, Willmitzer L, Frommer WB: Cis regulatory elements directing tuber specific and sucrose inducible expression of a class I patatin promoter. Mol Gen Genet 223: 401–406 (1990).Google Scholar
  24. 24.
    Höfgen R: Biochemische und genetische Untersuchungen zur Funktion des Patatinproteins in der Kartoffel (Solanum tuberosum). Ph.D. thesis, Free University of Berlin (1990).Google Scholar
  25. 25.
    Pikaard CS, Mignery GA, Ma DP, Stark VJ, Park WD: Sequence of two apparent pseudogenes of the major potato tuber protein, patatin. Nucl Acids Res 14: 5564–5566 (1986).Google Scholar
  26. 26.
    Köster-Töpfer M: Isolierung und funktionelle Characterisierung von verschiedenen Patatin Genen aus Solanum tuberosum. Ph.D. thesis, Free University of Berlin (1989).Google Scholar
  27. 27.
    Liu XY: Developmental- and tissue specific expression of chimeric class I and class II patatin genes in transgenic potato plants. Ph.D. thesis, Free University of Berlin (1989).Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Xiang-Yun Liu
    • 1
  • Mario Rocha-Sosa
    • 1
  • Sabine Hummel
    • 1
  • Lothar Willmitzer
    • 1
  • Wolf B. Frommer
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHBerlin 33FRG

Personalised recommendations