Photosynthesis Research

, Volume 5, Issue 2, pp 105–115

Adaptation of the thylakoid membranes of pea chloroplasts to light intensities. I. Study on the distribution of chlorophyll-protein complexes

  • Ta-Yan Leong
  • Jan M. Anderson
Article
  • 364 Downloads

Abstract

The effect of light intensity (16 h white light and 8 h dark) during growth of pea plants at 20°C on the chlorophyll composition and on the relative distribution of chlorophyll amongst the various chlorophyll-protein of pea thylakoids was studied. The chl a/chl b ratios increased from 2.1 to 3.2 as light intensity during growth varied from 10 to 840 μEm-2 s-1. This function can be described by two straight lines intersecting at a transition point of approximately 200 μEm-2 s-1. Similar discontinuities in the responses were observed in the changes in the relative distribution of chlorophyll amongst the various chlorophyll-protein complexes. This demonstrates that the chl a/chl b ratio of the various thylakoids is a good indicator of changes in the relative distribution of chlorophyll. As the chl a/chl b ratio decreased, the amount of chlorophyll associated with photosystem I complexes decreased, that with photosystem II core reaction centre complex was halved, and that with the main chl a/b-proteins of the light-harvesting complex was markedly increased.

Key words

chl a/chl b ratios chlorophyll-proteins light adaptation photosynthesis thylakoid membranes 

Abbreviations

chl

chlorophyll

PS

photosystem

SDS

sodium dodecyl sulphate

Tricine

N-tris (hydroxymethyl) methylglycine

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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1984

Authors and Affiliations

  • Ta-Yan Leong
    • 1
  • Jan M. Anderson
    • 1
  1. 1.Division of Plant IndustryCSIROCanberraAustralia

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