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Hydrobiologia

, Volume 188, Issue 1, pp 403–406 | Cite as

The Daphnia bioassay: a critique

  • Donald J. Baird
  • Ian Barber
  • Mairead Bradley
  • Peter Calow
  • Amadeu M. V. M. Soares
Article

Abstract

Daphnia magna is used widely as a standard ecotoxicological indicator organism, and protocols exist for its use in assessing the toxicity of substances under acute and chronic experimental conditions. Problems exist in repeatability of such bioassays between laboratories. Sources of variation are identified using a simple quantitative genetics model. Presenting specific examples, we conclude that these problems are tractable, but only if the genotype and culture conditions prior to and during tests are strictly controlled.

Key words

Daphnia bioassay effects of food concentration genetic variation maternal effects laboratory culture genotype-environment effects 

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References

  1. Adema, D. M. M., 1978. Daphnia magna as a test animal in acute and chronic toxicity tests. Hydrobiol. 59: 125–134.Google Scholar
  2. Berge, W. F. ten, 1978. Breeding Daphnia magna. Hydrobiol. 59: 121–123.Google Scholar
  3. Cabridenc, R., dy1986. Exercice d'intercalibration concernant une méthode de determination de l'ecotoxicité à moyen terme des substances chimiques vis-à-vis des daphnies. Unpublished EC Report. Contract W/63/476 (214). Ref. I.R.C.H.A.D. 8523. Vert-le-Petit, France. 20 pp.Google Scholar
  4. Falconer, D. S., 1981. Introduction to quantitative genetics. Longman. Harlow, Essex. 340 pp.Google Scholar
  5. Goulden, C. E., R. M. Comotto, J. A. Hendrickson Jr., L. L. Horning & K. L. Johnson, 1982. Procedures and recommendations for the culture and use of Daphnia in bioassay studies. Pages 139–160 in Proceedings of the American Society for Testing and Materials Fifth Toxicology Symposium, Special Technical Publication Number 7668, Philadelphia, Pennsylvania, USA.Google Scholar
  6. Hebert, P. D. N. & M. Beaton, 1986. Cellulose-acetate gel electrophoresis. Unpublished ms. Univ. Windsor, Ontario. 34 pp.Google Scholar
  7. OECD., 1981. Daphnia sp. 14 day reproduction test (including acute immobilisation test). OECD guidelines for testing of Chemicals, no. 202. ISBN 92-64-1221-4. Paris. 15 pp.Google Scholar
  8. Tessier, A. J., L. L. Henry, C. E. Goulden & M. W. Durand, 1983. Starvation in Daphnia: Energy reserves and reproductive allocation. Limnol. Oceanogr. 28: 667–676.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Donald J. Baird
    • 1
  • Ian Barber
    • 1
  • Mairead Bradley
    • 1
  • Peter Calow
    • 1
  • Amadeu M. V. M. Soares
    • 1
  1. 1.Department of Animal and Plant SciencesUniversity of SheffieldSheffieldU.K.

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