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Plant Molecular Biology

, Volume 11, Issue 3, pp 321–334 | Cite as

Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new class of seed proteins: cDNA cloning and characterization of the primary translation product

  • Ronald Bassüner
  • Helmut Bäumlein
  • Antje Huth
  • Rudolf Jung
  • Ulrich Wobus
  • Tom A. Rapoport
  • Gerhard Saalbach
  • Klaus Müntz
Article

Abstract

cDNA clones have been constructed bearing inserts for a specific mRNA class of high abundance in developing seeds of field bean (Vicia faba L.). Three full-length clones representing transcripts of different genes were sequenced and conceptually translated into a M=30 000 primary gene product. The structural analysis of the derived amino acid sequence revealed distinct domains: (i) a cleavable signal peptide; (ii) a hydrophilic N-terminal stretch possessing two serine clusters; (iii) a valine cluster and a hydrophobic domain in the C-terminal part of the polypeptide. The amino acid sequence of the polypeptide does not show homology with other known proteins.

The corresponding mRNA could be isolated using cDNA clones and was efficiently translated in various systems. In a cell-free system the presence of a functional signal peptide was shown, which interacts with the signal recognition particle resulting in a cotranslational translocation across the membrane of the endoplasmic reticulum. If synthesized in Xenopus oocytes the translation product of the mRNA was secreted out of the cell. Homologous mRNA was found to be present also in developing cotyledons of pea (Pisum sativum L.) and french bean (Phaseolus vulgaris L.).

Key words

field bean (Vicia faba L.) seed ontogenesis a class of predominating mRNA cDNA sequence seed protein signal peptide processing 

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Ronald Bassüner
    • 2
    • 1
  • Helmut Bäumlein
    • 2
    • 1
  • Antje Huth
    • 3
    • 1
  • Rudolf Jung
    • 2
    • 1
  • Ulrich Wobus
    • 2
    • 1
  • Tom A. Rapoport
    • 3
    • 1
  • Gerhard Saalbach
    • 2
    • 1
  • Klaus Müntz
    • 2
    • 1
  1. 1.Akademie der Wissenschaften der DDRGaterslebenGerman Democratic Republic
  2. 2.Zentralinstitut für Genetik und KulturpflanzenforschungGaterslebenGerman Democratic Republic
  3. 3.Zentralinstitut für MolekularbiologieBerlin-BuchGerman Democratic Republic

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