Plant Molecular Biology

, Volume 6, Issue 2, pp 111–117 | Cite as

Comparison between the organization of nuclear ribosomal DNA unit of Euglena gracilis Z and var. Bacillaris

  • Odile Neyret-Djossou
  • Georges Freyssinet
  • Patrick Ravel-Chapuis
  • Philippe Heizmann
Article
Received:
Revised:
Accepted:

Summary

We have characterized the nuclear rDNA unit of Euglena gracilis var. bacillaris and compared it to that of the Z strain. We have localized restriction sites for Eco R1, Sal 1, Sma 1, Hind III, Bam H1 and Bgl II on this unit as well as the coding region for 20 S and 25 S rRNAs. For both strains, results suggest an homogeneity of the 11.6 kbp rDNA units. Comparison between strains shows differences characterized by two additional Sal 1 sites in bacillaris and the likely methylation of one Sma 1 site in Z. Both differences are localized in a non-coding region of the rDNA unit. Analyses of 18 Euglena strains from various origins confirm these differences and allow easy recognition of bacillaris and Z type strains.

Keywords

nuclear rDNA Euglena 

Abbreviations

kb

kilo base

kpb

kilo base pair

plasmids

pRH 59 and pRH 57 contain a Hind III-HInd III nuclear DNA fragment from W3BUL of 5.9 and 5.7 kbp respectively, pRB 48 and pRB 35 contain a Bam H1-Bam H1 nuclear DNA fragment from wild-type Z of 4.8 and 3.5 kbp respectively

SDS

sodium dodecyl sulfate

UV

ultra-violet

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Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • Odile Neyret-Djossou
    • 1
    • 2
  • Georges Freyssinet
    • 1
    • 2
  • Patrick Ravel-Chapuis
    • 3
  • Philippe Heizmann
    • 3
  1. 1.Groupe de Recherche en Biochimie-Biologie CellulaireUniversité de limogesLimoges CédexFrance
  2. 2.Applications Laboratoire de Biologie ExpérimentaleUniversité de LimogesLimoges CédexFrance
  3. 3.Département de Biologie Générale et Appliquée, UA CNRS 92Université Lyon IVilleurbanne CédexFrance

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