Plant Molecular Biology

, Volume 19, Issue 4, pp 699–703

A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II

  • Jakob Brandt
  • Vibeke Skovgaard Nielsen
  • Hans Thordal-Christensen
  • David John Simpson
  • Jens Sigurd Okkers
Update Section Short Communication

Abstract

The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LHCII) in barley is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.

Key words

Type III LHCII CAB protein nucleotide sequence protein import light induction Hordeum vulgare 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Jakob Brandt
    • 1
  • Vibeke Skovgaard Nielsen
    • 2
  • Hans Thordal-Christensen
    • 1
  • David John Simpson
    • 3
  • Jens Sigurd Okkers
    • 2
  1. 1.Section for Plant Pathology, Department of Plant BiologyThe Royal Veterinary and Agricultural UniversityFredriksberg CDenmark
  2. 2.Plant Biochemistry Laboratory, Department of Plant BiologyThe Royal Veterinary and Agricultural UniversityFredriksberg CDenmark
  3. 3.Department of PhysiologyCarlsberg LaboratoryValbyDenmark

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