Identification and characterization of a hypoxically induced maize lactate dehydrogenase gene
In cereal root tissue, hypoxia induces the enzyme lactate dehydrogenase (LDH); (S)-lactate:NADH oxidoreductase, EC 184.108.40.206). In barley, both biochemical and genetic data indicate that five isozymes are induced under hypoxia. These isozymes are tetramers and arise from the random association of the products of two Ldh genes. The induction of LDH activity in root tissue has been shown to be correlated to an increase in LDH protein and Ldh mRNA.
In order to more fully characterize the hypoxic induction of LDH, we have isolated a maize Ldh genemic clone which has strong homology at both the amino acid and nucleotide level to the barley LDH cDNA clones. The Ldh1 gene consists of two exons separated by a 296 bp intron, has the expected eukaryotic regulatory signals and a sequence that has strong homology to the maize anaerobic regulatory element.
Key wordsmaize hypoxia lactate dehydrogenase anaerobic regulatory element
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- 1.Davies DD: Anaerobic metabolism and the production of organic acids. In: Davies DD (ed) The Biochemistry of Plants, Vol 2, pp. 581–611. Academic Press, New York (1980).Google Scholar
- 4.Gerlach WL, Pryor AJ, Dennis ES, Ferl RJ, Sachs MS, Peacock WJ. cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci USA 79: 2981–2985 (1982).Google Scholar
- 5.Good AG, Crosby WL. Induction of alcohol dehydrogenase and lactate dehydrogenase in hypoxically induced barley. Plant Physiol 90: 860–866 (1989).Google Scholar
- 6.Good AG, Crosby WL. Anaerobic induction of alanine aminotransferase in barley root tissue. Plant Physiol 90: 1305–1309 (1989).Google Scholar
- 9.Hoffman NE, Bent AF, Hanson AD: Induction of lactate dehydrogenase isozymes by oxygen deficit in barley root tissue. Plant Physiol 82: 658–663 (1986).Google Scholar
- 10.Hoffman NE, Hondred D, Hanson AD, Brown AHD: Lactate dehydrogenase isozymes in barley: polymorphism and genetic basis. J Hered 79: 110–114 (1988).Google Scholar
- 17.McElfresh KC, Chourey PS: Anaerobiosis induces transcription but not translation of sucrose synthase in maize. Plant Physiol 87: 542–546 (1988).Google Scholar
- 21.Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).Google Scholar
- 22.Walker JC, Howard EA, Dennis ES, Peacock WJ: DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci USA 84: 6624–6628 (1987).Google Scholar
- 23.Walker JC, Llewellyn DJ, Mitchell LE, Dennis ES: Anaerobically regulated gene expression; molecular adaptations of plants for survival under flooded conditions. Oxford Surv Plant Mol Cell Biol 4: 71–93 (1987).Google Scholar