Plant Molecular Biology

, Volume 19, Issue 4, pp 611–622

The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene

  • Wyatt Paul
  • Rachel Hodge
  • Sarah Smartt
  • John Draper
  • Rod Scott
Research Articles

DOI: 10.1007/BF00026787

Cite this article as:
Paul, W., Hodge, R., Smartt, S. et al. Plant Mol Biol (1992) 19: 611. doi:10.1007/BF00026787

Abstract

The Brassica napus cDNA clone A9 and the corresponding Arabidopsis thaliana gene have been sequenced. The B. napus cDNA and the A. thaliana gene encode proteins that are 73% identical and are predicted to be 10.3 kDa and 11.6 kDa in size respectively. Fusions of an RNase gene and the reporter gene β-glucuronidase to the A. thaliana A9 promoter demonstrated that in tobacco the A9 promoter is active solely in tapetal cells. Promoter activity is first detectable in anthers prior to sporogenous cell meiosis and ceases during microspore premitotic interphase.

The deduced A9 protein sequence has a pattern of cysteine residues that is present in a superfamily of seed plant proteins which contains seed storage proteins and several protease and α-amylase inhibitors.

Key words

anther Arabidopsis thaliana Brassica napus tapetum 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Wyatt Paul
    • 1
  • Rachel Hodge
    • 1
  • Sarah Smartt
    • 1
  • John Draper
    • 1
  • Rod Scott
    • 1
  1. 1.Department of BotanyLeicester UniversityLeicesterUK

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