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Dark-induced accumulation of a basic pathogenesis-related (PR-1) transcript and a light requirement for its induction by ethylene

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Abstract

A gene encoding a basic-type pathogenesis-related protein from Nicotiana tabacum (prb-1b) was cloned, sequenced and characterized. It contains an open reading frame of 179 amino acids that is ca. 65% homologous with the acidic PR-1 class of pathogenesis-related proteins and 87% homologous with a different basic-type PR-1 gene. In the light, physiological levels of ethylene rapidly (1 h) induced basic, but not acidic-type, PR-1 transcript. Additional elicitors acting via ethylene, such as α-aminobutyric acid, were shown to induce basic- and acidic-type PR-1 transcript accumulation in a light-dependent manner. In contrast, xylanase, an ethylene-independent elicitor, induced transcript accumulation of basic- and acidic-type PR-1 in a light-independent manner. Dark-induced accumulation of basic PR-1 transcript occurred at night in greenhouse-grown plants and, to a greater extent, in continuously dark-treated plants. The novel dark regulation may point to additional nonpathogenesis-related roles for these genes in plant-environment interactions.

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Authors and Affiliations

  1. Department of Plant Genetics, The Weizmann Institute of Science, P.O. Box 26, 76100, Rehovot, Israel

    Yoram Eyal & Robert Fluhr

  2. Department of Citriculture, Agricultural Research Organization, The Volcani Center, 50250, Bet-Dagan, Israel

    Oded Sagee

Authors
  1. Yoram Eyal
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  2. Oded Sagee
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  3. Robert Fluhr
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Eyal, Y., Sagee, O. & Fluhr, R. Dark-induced accumulation of a basic pathogenesis-related (PR-1) transcript and a light requirement for its induction by ethylene. Plant Mol Biol 19, 589–599 (1992). https://doi.org/10.1007/BF00026785

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  • DOI: https://doi.org/10.1007/BF00026785

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