Plant Growth Regulation

, Volume 9, Issue 1, pp 9–17 | Cite as

Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons

  • Chellathai Jayabaskaran
Article

Abstract

A protein which binds specifically to [3H]-zeatin has been isolated from cucumber cotyledons by chromatographic techniques. Its binding to [3H]-zeatin was inhibited remarkably by the addition of non-radioactive cytokinins and the order of inhibition was zeatin > -zeatin riboside > N6-(Δ2-isopentenyl)adenine > N6-(Δ2-isopentenyl)adenosine > N6-benzyl-adenosine > kinetin riboside. This protein behaved as a soluble protein with an apparent molecular size of 43,000 daltons on gel filtration through calibrated Sephadex G-100 column. The dissociation constant, Kd, of the protein-zeatin complex was about 4 × 10−7 M.

Key words

Cucumis sativus binding protein cytokinins and specificity 

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References

  1. 1.
    Armstrong, DJ, Burrows, WJ, Evans, PK andSkoog, F (1969) Isolation of cytokinins from tRNA. Biochem Biophys Res Commun 37: 451–456PubMedGoogle Scholar
  2. 2.
    Berridge, MV, Ralph, RK and Letham, DS (1970) The binding of kinetin to ribosomes. Biochem J 119: 75–84PubMedGoogle Scholar
  3. 3.
    Chen, CM, Melitz, DK, Petschow, B and Eckert, RL (1980) Isolation of cytokinin binding protein from plant tissues by affinity chromatography. Eur J Biochem 108: 379–387PubMedGoogle Scholar
  4. 4.
    Erion, JL and Fox, JE (1981) Purification and properties of a protein which binds cytokinin-active 6-substituted purines. Plant Physiol 67: 156–162Google Scholar
  5. 5.
    Fox, JE and Erion, JL (1975) A cytokinin binding protein from higher plant ribosomes. Biochem Biophys Res Commun 64: 695–700Google Scholar
  6. 6.
    Keim, P, Erion, J and Fox, JE (1981) The current status of cytokinin-binding moieties. In: JGuern and CPéaud-Lenoël eds. Metabolism and Molecular Activities of Cytokinins, 179–190. Berlin, Heidelberg, New York: Springer-VerlagGoogle Scholar
  7. 7.
    Lowry, OH, Rosebrough, NJ, Farr, AL and Randall, RJ (1951) Protein measurement with the folin phenol reagent. J. Biol Chem 193: 265–275PubMedGoogle Scholar
  8. 8.
    Matthysse, AG and Abrams, M (1970) A factor mediating interaction of kinins with the genetic material. Biochim Biophys Acta 199: 511–518PubMedGoogle Scholar
  9. 9.
    Moore, FH (1979) A cytokinin-binding protein from wheat germ. Plant Physiol 64: 594–599Google Scholar
  10. 10.
    Polya, GM and Davis, AW (1978) Properties of high affinity cytokinin-binding protein from wheat germ. Planta 139: 139–147Google Scholar
  11. 11.
    Polya, GM and Bowman, JA (1979) Ligand specificity of high affinity cytokinin-binding protein. Plant Physiol 64: 387–392Google Scholar
  12. 12.
    Ramanov, GA, Taran, VYa, Chvojka, L and Kulaeva, ON (1988) Receptor-like cytokinin-binding proteins from barley leaves. J Plant Growth Regul 7: 1–17Google Scholar
  13. 13.
    Reddy, ASN, Sopory, SK and Datta, A (1983) Purification and characterization of a cytokinin-binding protein from barley embryos. Biochem Int 6: 181–190Google Scholar
  14. 14.
    Scatchard, G (1949) The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672Google Scholar
  15. 15.
    Sussman, MR and Kende, H (1978) In vitro cytokinin binding to a particulate fraction of tobacco cells. Planta 140: 251–259Google Scholar
  16. 16.
    Yoshida, K and Takegami, T (1977) Isolation of cytokinin-binding protein from tobacco leaves by bioaffinity chromatography and its partial characterization. J Biochem 81: 791–799PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Chellathai Jayabaskaran
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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