Plant and Soil

, Volume 155, Issue 1, pp 33–43 | Cite as

Recent developments in electron microscopical techniques for studying ion localization in plant cells

  • Ralf Stelzer
  • Heiner Lehmann
Article

Abstract

This paper reviews recent technical approaches to the study of element localization in plant cells. It is concerned with sample preparation; with the use of electron probe microanalysis in the low temperature scanning electron microscope; with the use of electron energy loss spectrocopy and electron spectroscopic imaging in the transmission electron microscope. Basic principles of instrumentation, special problems during cryopreparation of plant tissues, and the application of these techniques within selected fields of botanical interests are briefly discussed.

Key words

EELS ESI freezing techniques high pressure freezing Ion localization LTSEM X-ray microanalysis 

Abbreviations

EDXA

Energy Dispersive X-Ray Analysis

EELS

Electron Energy Loss Spectrometry

EPMA

Electron Probe Microanalysis

ESEM

Environmental Scanning Electron Microscope

ESI

Electron Spectroscopic Imaging

HPF

High Pressure Freezing

LTSEM

Low Temperature Scanning Electron Microscope

SEM

Scanning Electron Micrsocope

TEM

Transmission Electron Microscope

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© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Ralf Stelzer
  • Heiner Lehmann

There are no affiliations available

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