Advertisement

Plant Growth Regulation

, Volume 14, Issue 3, pp 187–192 | Cite as

Methyl jasmonate and bean leaf abscission

  • Sara Jane Hall
  • Roger F. Horton
Article

Abstract

The effect of rac-methyl jasmonate, both in solution and as a vapour, on the separation of pulvinar and petiolar tissues in explants containing the distal abscission zone of primary leaves of Phaseolus vulgaris var. Contender was investigated. The effects of rac-methyl jasmonate were compared to those of (±)-abscisic acid, α-naphthalene acetic acid, ethylene and 2-chloroethylphosphonic acid. Abscission times were determined in explants prepared from 14-day-old control plants and in explants prepared from plants that had been pretreated for 24h with the ethylene-action inhibitor, silver thiosulphate. While silver-pretreatment, or treatment with α-naphthalene acetic acid delayed abscission, treatment with ethylene or 2-chloroethylphosphonic acid accelerated tissue separation. However, (±)-abscisic acid delayed abscission under these conditions. In all instances, treatment with rac-methyl jasmonate had no apparent effect on abscission. The loss of chlorophyll from bean leaf discs incubated in the dark was enhanced by treatment with 2-chloroethylphosphonic acid or (±)-abscisic acid and was retarded in discs incubated in benzyl adenine. While incubation in α-naphthalene acetic acid was without effect, incubation in solution of rac-methyl jasmonate also retarded chlorophyll loss when compared to water controls.

Key words

abscission chlorophyll loss ethylene methyl jasmonate Phaseolus vulgarissilver thiosulphate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Becker W and Apel K (1993) Differences in gene expression between natural and artificially induced leaf senescence. Planta 189: 74–79Google Scholar
  2. 2.
    Bourgouin NC and Horton RF (1992) Heterophylly in amphibious Ranunculi: the effects of abscisic acid and methyl jasmonate. In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in plant growth regulation, pp 386–392. Kluwer Acad Publ. DordrechtGoogle Scholar
  3. 3.
    Curtis RW (1984) Abscission-inducing properties of methyl jasmonate, ABA and ABA-methyl ester and their interactions with ethephon, Ag NO3 and malformin. Plant Growth Regul 3: 157–168Google Scholar
  4. 4.
    Falkenstein E, Groth B, Mithöfer A and Weiler EW (1991) Methyljasmonate and α-linolenic acid are potent inducers of tendril coiling. Planta 185: 316–322Google Scholar
  5. 5.
    Farmer EE and Ryan CA (1990) Interplant communications: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci USA 87: 7713–7716Google Scholar
  6. 6.
    Hamberg M and Gardner HW (1992) Oxylipin pathway to jasmonates: biochemistry and biological significance. Biochim Biophys Acta 1165: 1–18Google Scholar
  7. 7.
    Hermann G, Lehmann J, Peterson A, Sembdner G, Weidhase RA and Parthier B (1989) Species and tissue specificity of jasmonate-induced abundant proteins. J Plant Physiol 134: 703–709Google Scholar
  8. 8.
    Horton RF and Bourgouin NC (1992) Leaf senescence in juvenile ivy. Plant Physiol Biochem 30: 119–122Google Scholar
  9. 9.
    Liu JL, Mukherjee I and Reid DM (1990) Adventitious rooting in hypocotyls of sunflower (Helianthus annuus) seedlings. III. The role of ethylene. Physiol Plant 78: 268–276Google Scholar
  10. 10.
    Mason HS, DeWald DB, Creelman RA and Mullet JE (1992) Coregulation of soybean vegetative storage protein gene expression by methyl jasmonate and soluble sugars. Plant Physiol 98: 859–867Google Scholar
  11. 11.
    Nooden LD (1988) Abscisic acid, auxin and other regulators of senescence. In: Nooden LD and Leopold AC (eds) Senesence and aging in plants, pp 330–368. Academic Press, New YorkGoogle Scholar
  12. 12.
    Okhuma K, Addicott FT, Smith OE and Thiessen WE (1965) The structure of abscisin II. Tetrahedron Lett 29: 2529–2935Google Scholar
  13. 13.
    Osborne DJ (1989) Abscission. Crit Rev Plant Sci 8: 103–129Google Scholar
  14. 14.
    Parthier B (1990) Jasmonates: hormonal regulators or stress factors in leaf senescence? Plant Growth Regul 9: 57–63Google Scholar
  15. 15.
    Staswick PE (1992) Jasmonate, genes and fragrant signals. Plant Physiol 99: 804–807Google Scholar
  16. 16.
    Ueda J, Mizumto T and Kato J (1991) Quantitative changes of abscisic acid and methyl jasmonate correlated with vernal leaf abscission of Ficus superba var. japonica. Biochem Physiol Pflanz 187: 203–120Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Sara Jane Hall
    • 1
  • Roger F. Horton
    • 1
  1. 1.Department of BotanyUniversity of GuelphGuelphCanada

Personalised recommendations