Plant Molecular Biology

, Volume 25, Issue 1, pp 43–57

Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression

  • Marie-Françoise Gautier
  • Marie-Elisabeth Aleman
  • Anne Guirao
  • Didier Marion
  • Philippe Joudrier
Research Articles

Abstract

From a mid-maturation seed cDNA library we have isolated cDNA clones encoding two Triticum aestivum puroindolines. Puroindoline-a and puroindoline-b, which are 55% similar, are basic, cystine-rich and tryptophan-rich proteins. Puroindolines are synthezised as preproproteins which include N- and C-terminal propeptides which could be involved in their vacuolar localization. The mature proteins have a molecular mass of 13 kDa and a calculated isoelectric point greater than 10. A notable feature of the primary structure of puroindolines is the presence of a tryptophan-rich domain which also contains basic residues. A similar tryptophan-rich domain was found within an oat seed protein and a mammalian antimicrobial peptide. The ten cysteine residues of puroindolines are organized in a cysteine skeleton which shows similarity to the cysteine skeleton of other wheat seed cystine-rich proteins. Northern blot analysis showed that puroindoline genes are specifically expressed in T. aestivum developing seeds. No puroindoline transcripts as well as no related genes were detected in Triticum durum. The identity of puroindolines to wheat starch-granule associated proteins is discussed as well as the potential role of puroindolines in the plant defence mechanism.

Key words

cDNA sequence cystine-rich proteins gene expression puroindolines tryptophan-rich domain wheat 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Marie-Françoise Gautier
    • 1
  • Marie-Elisabeth Aleman
    • 1
  • Anne Guirao
    • 1
  • Didier Marion
    • 2
  • Philippe Joudrier
    • 1
  1. 1.Laboratoire de Biochimie et Biologie Moléculaire des CéréalesINRAMontpellier Cédex 01France
  2. 2.Laboratoire de Biochimie et Technologie des ProtéinesINRANantes Cédex 03France

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