, Volume 76, Issue 1–2, pp 101–106 | Cite as

Stability of bacterial leaf spot resistance in peach regenerants under in vitro, greenhouse and field conditions

  • F. A. Hammerschlag
  • D. J. Werner
  • D. F. Ritchie


Phenotypic stability of bacterial leaf spot resistance in peach (Prunus persica (L.) Batsch) regenerants, either selected at the cellular level for insensitivity to a toxic culture filtrate of Xanthomonas campestris pv. pruni or screened at the whole plant level for resistance to X. campestris pv. pruni, was investigated. A detached-leaf bioassay was used to evaluate the original regenerants again after three years in the greenhouse and also after a two to three year cycle of tissue culture propagation. Peach trees derived through micropropagation from the original regenerants were also evaluated after one to three years growth in the field. Although leaf spot resistance was retained in some regenerants over time in the greenhouse, following in vitro propagation, and under field conditions, resistance was either lost or not expressed in others. Regenerants # 19-1 and #156-6, derived from embryo callus of bacterial spot susceptible ‘Sunhigh’, were significantly more resistant than ‘Sunhigh’. High levels of resistance were exhibited in greenhouse plants and field-grown trees of regenerant #122-1, derived from embryo callus of moderately resistant ‘Redhaven’. This research provides additional evidence that selecting or screening for somaclonal variants with disease resistance is a feasible approach to obtaining peach trees with increased levels of bacterial spot resistance.

Key words

in vitro selection Prunus persica somaclonal variation Xanthomonas campestris pv. pruni bacterial leaf spot of peach 





Toxic culture Filtrate


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • F. A. Hammerschlag
    • 1
  • D. J. Werner
    • 2
  • D. F. Ritchie
    • 3
  1. 1.Plant Molecular Biology LaboratoryU.S. Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.Department of Horticultural ScienceNorth Carolina State UniversityRaleighUSA
  3. 3.Department of Plant PathologyNorth Carolina State UniversityRaleighUSA

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