Summary
The classical genetic map and molecular map of rice chromosome 11 were oriented to facilitate the use of these maps for genetic studies and rice improvement. Three morphological markers (d-27, z-2, and la) were crossed to a rice breeding line, IRBB21, which has the Xa-21 gene for bacterial blight resistance. Three F2 populations were analyzed with RFLP markers known to be located on chromosome 11. Segregation analysis of molecular markers and morphological markers was used to construct an RFLP map for each population. The recombination frequency between markers varied from population to population although the marker order on the maps was the same for all three populations. Based on a common set of markers mapped in the three populations, an integrated map was generated consisting of both RFLP and morphological markers. The genetic distance between markers on this map was determined by taking a weighted average of the data from the three populations. The oriented map serves as a bridge to understand the relationship between the classical and molecular linkage maps. Based on this information, the location of several genes on the classical map can be approximated with respect to RFLP markers without having to map them directly.
Similar content being viewed by others
References
Dellaporta S.L., J.Wood & J.B.Hicks, 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1: 19–21.
Feinberg A.P. & B.Vogelstein, 1983. A technique for radiola-belling DNA restriction fragments to a high specific activity. Anal. Biochem. 132: 6–13.
Ideta, O., A. Yoshimura, H. Tsunematsu, T. Matsumoto & N. Iwata, 1992. Integration of RFLP and conventional linkage maps in rice. In: Proceedings of the Asia-Pacific Conference on Agricultural Biotechnology, pp. 163–166. Beijing, China.
Ikeda R., G.S.Khush, R.E.Tabien & T.Ogawa, 1990. A new resistance gene to bacterial blight from Oryza longistaminata. Jpn. J. Breed. 40: 280–281.
Ikeda R., R.E.Tabien & G.S.Khush, 1991. Chromosomal location of Xa-21. Rice Genet. Newsl. 8: 102–103.
Inukai T., D.J.Mackill, J.M.Bonman, S.Sarkarung, R.Zeigler, R.Nelson, I.Takamure & T.Kinoshita, 1992. Blast resistance genes Pi-2(t) and Pi-z may be allelic. Rice Genet. Newsl. 9: 90–92.
Ishikawa R., H.Morishima, T.Kinoshita, T.Harada, M.Niizeki & K.Saito, 1991. Linkage analysis of nine isozyme genes on the conventional linkage map in rice. Jpn. J. Breed. 41: 265–272.
Iwata N. & T.Omura, 1977. Linkage studies in Rice (Oryza sativa L). On some mutants derived from chronic gamma irradiation. J. Fac. Agric. Kyushu Univ. 21: 117–127.
Iwata N., T.Omura & H.Satoh, 1978. Linkage studies in rice. The sequence of genes at the eighth and eleventh linkage groups. Jpn. J. Breed. 28: 170–171.
Kauffman H.E., A.P.K.Reddy, S.P.Y.Hsien & S.D.Merca, 1973. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis. Rep. 57: 537–541.
Khush G.S., E.Bacalangco & T.Ogawa, 1990. A new gene for resistance to bacterial blight from O. longistaminata. Rice Genet. Newsl. 7: 121–122.
Khush G.S. & T.Kinoshita, 1991. Rice Karyotype, Marker Genes, and Linkage Groups. In: G.S.Khush & G.H.Toenniessen (Eds.), Rice Biotechnology, pp. 83–108. CAB International and IRRI, Wallingford, Oxon, UK and Manila, Philippines.
Kinoshita T., 1990. Report of the committee on gene symbolization, nomenclature and linkage groups. Rice Genet. Newsl. 7: 16–57.
Kishimoto, N., E. Shimosaka, R. Ohsawa & A. Saito, 1992. Correlation of the current rice RFLP map with the classical genetic map. In: Proceedings of the Asia-Pacific Conference on Agricultural Biotechnology, pp. 246–248. Beijing, China.
Lander E.S., P.Green, J.Abrahamson, A.Barlow, M.J.Daly, S.E.Lincoln & L.Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.
McCouch S.R., G.Kochert, Z.H.Yu, Z.Y.Wang, G.S.Khush, W.R.Coffman & S.D.Tanksley, 1988. Molecular mapping of rice chromosomes. Theor. Appl. Genet. 76: 815–829.
McCouch S.R. & S.D.Tanksley, 1991. Development and Use of Restriction Fragment Length Polymorphism in Rice Breeding and Genetics. In: G.S.Khush & G.H.Toenniessen (Eds.), Rice Biotechnology, pp. 109–133. CAB International and IRRI, Wallingford, Oxon, UK and Manila, Philippines.
Nagao S. & T.Takahashi, 1963. Trial construction of twelve linkage groups in Japanese rice. Genetical studies on rice plant XXVII. J. Fac. Agric. Hokkaido Univ. 53: 72–130.
Reiter R.S., J.G.K.Williams, K.A.Feldmann, J.A.Rafalski, S.V.Tingey & P.A.Scolnik, 1992. Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs. Proc. Natl. Acad. Sci. USA 89: 1477–1481.
Ronald P.C., B.Albano, R.Tabien, L.Abenes, K.Wu, S.McCouch & S.Tanksley, 1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa-21. Mol. Gen. Genet. 236: 113–120.
Saito A., M.Yano, N.Kishimoto, M.Nakagahra, A.Yoshimura, K.Saito, S.Kuhara, Y.Ukai, M.Kawase, T.Nagamine, S.Yoshimura, O.Ideta, R.Ohsawa, Y.Hayano, N.Iwata & M.Sugiura, 1991. Linkage map of restriction fragment length polymorphism loci in rice. Jpn. J. Breed. 41: 665–670.
Sambrook J., E.Fritsch & T.Maniatis, 1989. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Tai T.H. & S.D.Tanksley, 1990. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol. Biol. Rep. 8: 297–303.
Tanksley S., M.Causse, T.Fulton, N.Ahn, Z.Wang, K.Wu, J.Xiao, Z.Yu, G.Second & S.McCouch, 1992. High density molecular map of the rice genome. Rice Genet. Newsl. 9: 111–115.
Wu K.S., J.C.Glaszmann & G.S.Khush, 1988. Chromosomal location of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis. Biochem. Genet. 26: 311–328.
Xiao J., T.Fulton, S.McCouch, S.Tanksley, N.Kishimoto, R.Ohsawa, Y.Ukai & A.Saito, 1992. Progress in integration of the molecular maps of rice. Rice Genet. Newsl. 9: 124–128.
Yoshimura S., A.Yoshimura, A.Saito, N.Kishimoto, M.Kawase, M.Yano, M.Nakagahra, T.Ogawa & N.Iwata, 1992. RFLP analysis of introgressed chromosomal segments in three near-isogenic lines of rice bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4. Jpn. J. Genet. 67: 29–37.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abenes, M.L.P., Tabien, R.E., McCouch, S.R. et al. Orientation and integration of the classical and molecular genetic maps of chromosome 11 in rice. Euphytica 76, 81–87 (1994). https://doi.org/10.1007/BF00024023
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024023