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Plant Molecular Biology

, Volume 16, Issue 3, pp 449–461 | Cite as

The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies

  • Michel A. Haring
  • Caius M. T. Rommens
  • H. John J. Nijkamp
  • Jacques Hille
Article

Abstract

This review compares the activity of the plant transposable elements Ac, Tam3, En/Spm and Mu in heterologous plant species and in their original host. Mutational analysis of the autonomous transposable elements and two-element systems have supplied data that revealed some fundamental properties of the transposition mechanism. Functional parts of Ac and En/Spm were detected by in vitro binding studies of purified transposase protein and have been tested for their importance in the function of these transposable elements in heterologous plant species. Experiments that have been carried out to regulate the activity of the Ac transposable element are in progress and preliminary results have been compiled. Perspectives for manipulated transposable elements in transposon tagging strategies within heterologous plant species are discussed.

Key words

transposition mechanism Ac Tam3 En/Spm Mu two-element systems transposon tagging transgenic plants 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Michel A. Haring
    • 1
  • Caius M. T. Rommens
    • 1
  • H. John J. Nijkamp
    • 1
  • Jacques Hille
    • 1
  1. 1.Dept. of GeneticsFree UniversityAmsterdamThe Netherlands

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