, Volume 85, Issue 1–3, pp 281–285 | Cite as

Sexual and somatic hybridization in the genus Lactuca

  • Brigitte Maisonneuve
  • Marie Christine Chupeau
  • Yannick Bellec
  • Yves Chupeau


Various genes for disease resistance identified in wild Lactuca are difficult, even impossible to exploit in lettuce breeding, due to sexual incompatibility between L. sativa and wild Lactuca sp. We adapted two cellular biology techniques to overcome these interspecific barriers: in vitro embryo rescue and protoplast fusion. In vitro rescue of immature embryos was used successfully for sexual hybridization between L. sativa and L. virosa. Vigorous hybrid plants were produced between L. sativa and seven accessions of L. virosa. Protoplast fusion permitted the regeneration of somatic hybrids between L. sativa and either L. tatarica or L. perennis. Hybrids between L. sativa and L. tatarica were backcrossed to L. sativa.

Key words

Lactuca sativa Lactuca virosa Lactuca tatarica Lactuca perennis Iettuce sexual hybridization embryo rescue somatic hybridization protoplast fusion 


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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Brigitte Maisonneuve
    • 1
  • Marie Christine Chupeau
    • 2
  • Yannick Bellec
    • 1
  • Yves Chupeau
    • 2
  1. 1.Station de Génétique et d' Amélioration des PlantesInstitut National de la Recherche AgronomiqueVersailles CedexFrance
  2. 2.Laboratoire de Biologie CellulaireInstitut National de la Recherche AgronomiqueVersailles CedexFrance

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