Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase
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Oxalic acid is thought to have a primary role in the pathogenicity of several plant pathogens, notably Sclerotinia selerotiorum. A gene coding for the enzyme oxalate oxidase was isolated from barley roots and introduced into oilseed rape as a means of degrading oxalic acid in vivo. This report describes the production of several transgenic plants of oilseed rape and the characterisation of these plants by Southern, Western and enzyme activity assays. Plants were shown to contain an active oxalate oxidase enzyme and were tolerant of exogenously supplied oxalic acid.
Key wordsBrassica napus disease tolerance oxalic acid oxalate oxidase Sclerotinia sclerotiorum transformation
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- Marciano P., Di Lenna & P. Magro, 1983. Oxalic acid, cell wall degrading enzymes and pH in pathogenesis and their significance in the virulence of two Sclerotinia sclerotiorum isolates on sunflower. Physiol. Plant Pathol. 22: 339–345.Google Scholar
- Noyes R.D. & J.G. Hancock, 1981. Role of oxalic acid in the Sclerotinia wilt of sunflower. Physiol. Plant Pathol. 18: 123–132.Google Scholar
- Schmitt M.R., 1991. Barley seedling oxalate oxidase: purification and properties. Plant Physiol. (suppl.) 96: 85.Google Scholar
- Sugiura M., H. Yamamura, K. Hirano, M. Sasaki, M. Morikawa & M. Tsuboi, 1979. Purification and properties of oxalate oxidase from barley seedlings. Chem. Pharm. Bull. 27: 2003–2007.Google Scholar