Euphytica

, Volume 85, Issue 1–3, pp 75–80 | Cite as

Maize transformation utilizing silicon carbide whiskers: a review

  • J. A. Thompson
  • P. R. Drayton
  • B. R. Frame
  • Kan Wang
  • J. M. Dunwell
Article

Summary

We review here the most recently developed technique for maize transformation which involves the vortexing of silicon carbide whiskers with maize cells in the presence of plasmid DNA. Fertile transgenic plants have been regenerated following whisker-mediated transformation which is compared with the alternatives described to date, namely protoplast uptake, particle bombardment and electroporation of intact tissue.

Key words

transformation silicon carbide whiskers maize 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Asano Y., Y. Otsuki & M. Ugaki, 1991. Electroporation-mediated and silicon carbide whisker-mediated DNA delivery in Agrostis alba L. (Redtop). Plant Science 9: 247–252.CrossRefGoogle Scholar
  2. Bennetzen J.L., C. Lin, S. McCormick & B.J. Staskawicz, 1988. Transformation of Adh null pollen to Adh+ by ‘macroinjection’. Maize Genetics Cooperative Newsletter 62: 113–114.Google Scholar
  3. Booy G., F.A. Krens & H.J. Huizing, 1989. Attempted pollenmediated transformation of maize. J. Plant Physiol. 135: 319–324.Google Scholar
  4. Coe E.H. & K.R. Sarkar, 1966. Preparation of nucleic acids and a genetic transformation attempt in maize. Crop Science 6: 432–435.CrossRefGoogle Scholar
  5. D'Halluin K., E. Bonne, M. Bossut, M.De Beuckeleer & J. Leemans, 1992. Transgenic maize plants by tissue electroporation. The Plant Cell 4: 1495–1505.PubMedCrossRefGoogle Scholar
  6. Dunahay T.G., 1993. Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. Bio Techniques 15: 452–460.Google Scholar
  7. Frame B.R., P.R. Drayton, S.V. Bagnall, C.J. Lewnau, W.P. Bullock, H.M. Wilson, J.M. Dunwell, J.A. Thompson & K. Wang, 1994. Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation. The Plant Journal 6: 941–948.CrossRefGoogle Scholar
  8. Fromm M.E., F. Morrish, C.L. Armstrong, R. Williams, J. Thomas & T.M. Klein, 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–839.PubMedCrossRefGoogle Scholar
  9. Fromm M.E., 1994. Production of transgenic maize plants by microprojectile-mediated gene transfer. In: M. Freeling & V. Walbot (Eds). The Maize Handbook, pp. 677–684. Springer-Verlag, New York.Google Scholar
  10. Gaillard A., E. Matthys-Rochon & C. Dumas, 1992. Selection of microspore derived embryogenic structures in maize related to transformation potential by microinjection. Bot. Acta 105: 313–318.Google Scholar
  11. Golovkin M.V., M. Abraham, S. Mórocz, S. Bottka, A. Fehér & D. Dudits, 1993. Production of transgenic maize plants by direct DNA uptake into protoplasts. Plant Science 90: 41–52.CrossRefGoogle Scholar
  12. Gordon-Kamm W.J., T.M. Spencer, M.L. Mangano, T.R. Adams, R.J. Daines, W.G. Start, J.V. O'Brien, S.A. Chambers, W.R. Adams, N.G. Willetts, T.B. Rice, C.J. Mackay, R.W. Krueger, A.P. Kausch & P.G. Lemaux, 1990. Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603–618.PubMedCrossRefGoogle Scholar
  13. Gould J., M. Devey, O. Hasegawa, E.C. Ulian, G. Peterson & R.H. Smith, 1991. Transformation of Zea mays L. using Agrobacterium tumefaciens and the shoot apex. Plant Physiol. 95: 426–434.PubMedCrossRefGoogle Scholar
  14. Graves A.C. & S.L. Goldman, 1986. The transformation of Zea mays seedlings with Agrobacterium tumefaciens. Plant Mol. Biol. 7: 43–50.CrossRefGoogle Scholar
  15. Greenwood N.N. & A. Earnshaw, 1984. Silicon carbide. In: Chemistry of the Elements, pp. 386–394. Pergamon Press, Oxford.Google Scholar
  16. Hunold R., R. Bronner & G. Hahne, 1994. Eearly events in microprojectile bombardment: cell viability and particle location. The Plant Journal 5: 593–604.CrossRefGoogle Scholar
  17. Kaeppler H.F., W. Gu, D.A. Somers, H.W. Rines & A.F. Cockburn, 1990. Silicon carbide fiber-mediated DNA delivery into plant cells. Plant Cell Rep. 9: 415–418.CrossRefGoogle Scholar
  18. Kaeppler H.F., D.A. Somers, H.W. Rines & A.F. Cockburn, 1992. Silicon carbide fiber-mediated stable transformation of plant cells. Theor. Appl. Genet. 84: 560–566.CrossRefGoogle Scholar
  19. Kaeppler H.F. & D.A. Somers, 1994. DNA delivery to maize cell cultures using silicon carbide fibers. In: M. Freeling & V. Walbot (Eds). The Maize Handbook, pp. 610–613. Springer-Verlag, New York.Google Scholar
  20. Kivilaan A. & D.F. Blaydes, 1974. Attempts to achieve genetic transformation in plants. Michigan State University Research Report 246: 2–5.Google Scholar
  21. Klein T.M., M. Fromm, A. Weissinger, D. Tomes, S. Schaaf, M. Sletten & J.C. Sanford, 1988. Transfer of foreign genes into intact maize cells using high velocity microprojectiles. Proc. Natl. Acad. Sci. USA 85: 4305–4309.PubMedCrossRefGoogle Scholar
  22. Klein T.M., L. Kornstein, M.E. Fromm, J.C. Sanford, 1989. Genetic transformation of maize cells by particle bombardment. Plant Physiol. 91: 440–444.PubMedCrossRefGoogle Scholar
  23. Konstantinov K., S. Mladenovic & M. Denic, 1991. Recombinant DNA technology in maize breeding. V. Plant and indigenous bacterial strains transformed by the gene controlling kanamycin resistance. Genetika (Beograd) 23: 121–135.Google Scholar
  24. Korohoda J. & K. Strzalka, 1979. High efficiency genetic transformation in maize induced by exogenous DNA. Z. Pflanzenphysiol. 94: 95–99.Google Scholar
  25. Koziel M.G., G.L. Beland, C. Bowman, N.B. Carozzi, R. Crenshaw, L. Crossland, J. Dawson, N. Desai, M. Hill, S. Kadwell, K. Launis, K. Lewis, D. Maddox, K. McPherson, M.R. Meghji, E. Merlin, R. Rhodes, G.W. Warren, M. Wright & S.V. Evola, 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology 11: 194–200.CrossRefGoogle Scholar
  26. Laursen C.M., R.A. Kryzek, C.E. Flick, P.C. Anderson & T.M. Spencer, 1994. Production of fertile transgenic maize by electroporation of suspension culture cells. Plant Mol. Biol. 24: 51–61.PubMedCrossRefGoogle Scholar
  27. Mutsuddy B.C., 1990. Electrokinetic behaviour of aqueous silicon carbide whisker suspensions. J. Am. Ceram. Soc. 9: 2747–2749.CrossRefGoogle Scholar
  28. Mórocz S., G. Donn, J. Nemeth & D. Dudits, 1990. An improved system to obtain fertile regenerants via maize protoplasts isolated from a highly embryogenic suspension culture. Theor. Appl. Genet. 80: 721–726.CrossRefGoogle Scholar
  29. Ohta Y., 1986. High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc. Natl. Acad. Sci. USA 83: 715–719.PubMedCrossRefGoogle Scholar
  30. Petersen W.L., S. Sulc & C.L. Armstrong, 1992. Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts. Plant Cell Rep. 10: 591–594.CrossRefGoogle Scholar
  31. Potrykus I., 1990. Gene transfer to plants: assessment and perspectives. Physiologia Plantarum 79: 125–134.CrossRefGoogle Scholar
  32. Prioli L.M. & M.R. Sondahl, 1989. Plant regeneration and recovery of fertile plants protoplasts of maize (Zea mays L.). Bio/Technology 7: 589–594.CrossRefGoogle Scholar
  33. Rasmussen J.L., J.R. Kikkert, M.K. Roy & J.C. Sanford, 1994. Biolistic transformation of tobacco and maize suspension cells using bacterial cells as microprojectiles. Plant Cell Rep. 13: 212–217.CrossRefGoogle Scholar
  34. Shillito R.D., G.K. Carswell, C.M. Johnson, J.M. DiMaio & C.T. Harms, 1989. Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technology 7: 581–587.CrossRefGoogle Scholar
  35. Spencer T.M., W.J. Gordon-Kamm, R.J. Daines, W.G. Start & P. Lemaux, 1990. Bialaphos selection of stable transformants from maize cell cultures. Theor. Appl. Genet. 79: 625–631.CrossRefGoogle Scholar
  36. Spencer T.M., J.V. O'Brien, W.G. Start, T.R. Adams, W.J. Gordon-Kamm & P.G. Lemaux, 1992. Segregation of transgenes in maize. Plant Mol. Biol. 18: 201–210.PubMedCrossRefGoogle Scholar
  37. Vain P., M.D. McMullen & J.J. Finer, 1993. Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize. Plant Cell Rep. 12: 84–88.CrossRefGoogle Scholar
  38. Wang, K., B.R. Frame, P.R. Drayton & J.A. Thompson, 1994. Silicon carbide whisker-mediated transformation: Regeneration of transgenic maize plants. In: I. Potrykus (Ed). Gene Transfer to Plants. Springer-Verlag, in press.Google Scholar
  39. Wang, K., P.R. Drayton, B.R. Frame, J.M. Dunwell & J.A. Thompson, 1994. Whisker-mediated plant transformation: An alternative technology. In Vitro Cell. Devel. Biol., in press.Google Scholar
  40. Wilson H.M., B.P. Bullock, J.M. Dunwell, J.R. Ellis, B.R. Frame, J.R. Register & J.A. Thompson, 1994. Maize transformation. In: K. Wang, A. Herrera-Estrella & M.Van Montagu (Eds). Transformation of Plants and Soil Microorganism, pp. 65–80. Cambridge University Press, Cambridge.Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • J. A. Thompson
    • 1
  • P. R. Drayton
    • 1
  • B. R. Frame
    • 2
  • Kan Wang
    • 2
  • J. M. Dunwell
    • 1
  1. 1.Plant BiotechnologyZENECA SeedsBracknell, BerkshireU.K.
  2. 2.ICI SeedsSlaterU.S.A.

Personalised recommendations