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Euphytica

, Volume 90, Issue 2, pp 245–250 | Cite as

Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry

  • R. Bošković
  • K. R. Tobutt
Article

Summary

Protein stylar extracts of 16 cultivars of sweet cherry (Prunus avium), from the 10 different incompatibility groups to which incompatibility alleles have been assigned, were separated on acrylamide gels using isoelectric focusing (IEF) and were stained for ribonuclease activity. When two cultivars from the same incompatibility group were analyzed they gave identical zymograms and the cultivars of the 10 different incompatibility groups gave in all eight distinct zymograms. The ribonuclease polymorphism could be correlated with the reported S allele constitutions of the cultivars. Three ribonuclease bands were identified that each consistently corresponded to one of the six known incompatibility alleles (S1, S2 and S6), a fourth band apparently corresponded to S3 and to the combination of S4 and S5, and a fifth band to S4 and S5 in other combinations. Thus, it seems that S alleles of cherry have ribonuclease activity and that IEF is useful for distinguishing S allele constitutions. The ribonuclease pattern of ‘Summit’, a cultivar of unknown incompatibility group, indicated its incompatibility genotype to be S1S2, and this was confirmed by controlled pollination. The same band corresponded to S4 and S4', the mutant allele in self-compatible cultivars. IEF and ribonuclease staining promise to be useful tools for exploring the incompatibility relationships of cherry cultivars and perhaps of other self-incompatible Prunus crops.

Key words

cherry incompatibility isoenzymes isoelectric focusing Prunus avium ribonuclease 

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References

  1. Brown, A.G., 1955. Department of Plant Breeding. Report of John Innes Horticultural Institution for 1954: 6–11.Google Scholar
  2. Crane M.B. & A.G. Brown, 1937. Incompatibility and sterility in the sweet cherry, Prunus avium L. Journal of Pomology & Horticultural Science 15: 80–116.Google Scholar
  3. Crane M.B. & W.J.C. Lawrence, 1929. Genetical and cytological aspects of incompatibility and sterility in cultivated fruits. Journal of Pomology & Horticultural Science 7: 276–301.Google Scholar
  4. Crane M.B. & W.J.C. Lawrence, 1931. Sterility and incompatibility in diploid and polyploid fruits. Journal of Genetics 24: 97–107.Google Scholar
  5. Kobel F., P. Steinegger & J. Anliker, 1938. Weitere Untersuchungen über die Befruchtungsverhältnisse der Kirschensorten. Landwirtschaftlichen Jahrbuch der Schweiz 52: 564–595.Google Scholar
  6. Lane W.D. & H. Schmid, 1984. Lapins and Sunburst sweet cherry. Canadian Journal of Plant Science 64: 211–214.Google Scholar
  7. Lapins K.O., 1973. Induced mutations in fruit trees. In: Induced Mutations in Vegetatively Propagated Plants, pp. 1–19. IAEA, Vienna.Google Scholar
  8. McClure B.A., V. Haring, P.R. Ebert, M.A. Anderson, R.J. Simpson, F. Sakiyama & A.E. Clarke, 1989. Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342: 955–957.Google Scholar
  9. Matthews P. & K.P. Dow, 1969. Incompatibility groups: sweet cherry (Prunus avium). In: R.L. Knight (Ed.). Abstract Bibliography of Fruit Breeding & Genetics to 1965, Prunus, pp. 540–544. Commonwealth Agricultural Bureaux, Farnham Royal.Google Scholar
  10. Matthews, P., 1970. The genetics and exploitation of self-fertility in the sweet cherry. Proceedings of the Angers Fruit Breeding Symposium, September 1970: 307–316.Google Scholar
  11. Mau S.L., J. Raff & A.E. Clarke, 1982. Isolation and partial characterisation of components of Prunus avium L. styles, including an antigenic glycoprotein associated with a self-incompatibility genotype. Planta 156: 505–516.Google Scholar
  12. Sansavini S. & S. Lugli, 1994. Il miglioramento genetico del ciliego dolce per l'autofertilià, l'habitus compatto e l'altra qualità del frutto. Rivista di Frutticoltura 56 (6): 19–27.Google Scholar
  13. Sassa H., H. Hirano & H. Ikehashi, 1992. Self-incompatibility-related RNases in styles of Japanese pear (Pyrus serotina Rehd.). Plant Cell Physiology 33: 811–814.Google Scholar
  14. Tehrani G. & W. Lay, 1991. Verification through pollen incompatibility studies of pedigrees of sweet cherry cultivars from Vineland. HortScience 26: 190–191.Google Scholar
  15. Tobutt & Bošković, 1994. Progress in constructing a linkage map for Prunus. Annual Report of Horticulture Research International 1993–94: 35.Google Scholar
  16. Way R.D., 1968. Pollen incompatibility groups of sweet cherry clones. Proceedings of the American Society for Horticultural Science 92: 119–123.Google Scholar
  17. Wilson C.M., 1971. Plant nucleases III. Polyacrylamide gel electrophoresis of com ribonuclease isoenzymes. Plant Physiology 48: 64–68.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • R. Bošković
    • 1
  • K. R. Tobutt
    • 1
  1. 1.Horticulture Research InternationalU.K.

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