Comparison of callus culture with embryo culture at different times of embryo rescue for primary triticale production
- 123 Downloads
Callus culture of immature wheat-rye hybrid embryos was compared with embryo culture in two experiments. Embryos were rescued from field grown mother plants at two day intervals 13–21 days after pollination and plated for 1) callus culture on Murashige and Skoog medium (MS) supplemented with 2 mg/12,4-dichlorophenoxy acetic acid followed by plant regeneration on hormone free MS medium with half strength mineral salts, 2) embryo culture on Taira and Larter medium (TL). Observations were made on embryo size and condition at time of rescue (Experiment 1) and embryo development directly into plants (embryo culture) or through embryogenesis (callus culture). Fewer 19 and 21 day old embryos developed into plants from callus culture than from embryo culture in Experiment 1. Callus culture was more efficient than embryo culture in promoting plant recovery from 17 day old embryos in Experiment 2. The number of plants per embryo was significantly higher from callus culture than from embryo culture. In both experiments callus culture promoted embryogenesis in more embryos than developed in embryo culture. Embryo rescue 15–17 days after pollination was optimal in both experiments.
Key wordscallus culture embryo culture immature embryo primary triticale wheat-rye hybrid
Unable to display preview. Download preview PDF.
- INSTAT, 1990. PC Teaching Guide, Statistical Services Centre. University of Reading, U.K.Google Scholar
- Kaltsikes P.J. & J.P. Gustafson, 1986. Triticale (Triticosecale): Production through embryo culture. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry. Vol. 2: Crops I, pp. 523–529. Springer-Verlag, Berlin, Heidelberg.Google Scholar
- Keyworth S. & E.N. Larter, 1979. Embryo and endosperm development in wheat-rye hybrids. Cereal Res. Comm. 7: 135–144.Google Scholar
- Merkle, O.G., A.C. Cuenzi, R.A. Veal & C.A. Baker, 1988. Comparison of embryo culture with callus induction and plant regeneration for recovering interspecific wheat hybrids. Agr. Abst. C-7, pp. 170–171.Google Scholar
- Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.Google Scholar
- Raina S.K., 1984. Crossability and in vitro development of hybrid embryos of Triticum durum × Secale cereale. Indian J. Genet. 44: 429–437.Google Scholar
- SAS User's Guide, 1985. Version 5 Edn., Cary, N.C., USA, SAS Institute.Google Scholar
- Shao Z.Z. & T. Taira, 1990. Production of primary triticale from calluses of immature abnormal hybrid embryos between Triticum durum and Secale cereale. Plant Breeding 105: 81–88.Google Scholar
- Taira T. & E.N. Larter, 1978. Factors influencing development of wheat-rye hybrid embryos in vitro. Crop Science 18: 348–350.Google Scholar
- Varughese G., T. Barker & E. Saari, 1987. Triticale. CIMMYT, Mexico, D.F. 32 pp.Google Scholar