Plant Molecular Biology

, Volume 21, Issue 6, pp 1109–1119 | Cite as

Transposition pattern of a modified Ds element in tomato

  • Caius M. T. Rommens
  • Tichafa R. I. Munyikwa
  • Bert Overduin
  • H. John J. Nijkamp
  • Jacques Hille
Research Articles
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Accepted:

Abstract

Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a ‘late’ timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that ‘genes to be tagged’ should be located in genomic regions covered by insertions.

Key words

transposition Ac/Ds transgenic tomato plants RFLP mapping plasmid rescue 
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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Caius M. T. Rommens
    • 1
  • Tichafa R. I. Munyikwa
    • 1
  • Bert Overduin
    • 1
  • H. John J. Nijkamp
    • 1
  • Jacques Hille
    • 1
  1. 1.Department of GeneticsFree UniversityAmsterdamNetherlands

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