Integration of CAPS markers into the RFLP map generated using recombinant inbred lines of Arabidopsis thaliana
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Konieczny and Ausubel have described a technique whereby Arabidopsis thaliana loci can be rapidly mapped to one of the ten chromosome arms using a small number of F2 progeny from crosses between the ecotypes Landsberg erecta and Columbia. The technique involves the use of 18 co-dominant, cleaved amplified polymorphic sequence (CAPS) markers which are evenly distributed throughout the Arabidopsis genome. We have mapped these 18 markers using recombinant inbred (RI) lines generated in our laboratory. These data enable a better integration of loci mapped relative to the CAPS markers into the restriction fragment length polymorphism (RFLP) map generated using Arabidopsis RI lines.
Key wordsArabidopsis CAPS markers rapid mapping recombinant inbred lines
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- 2.Dean C, Sjodin C, Page T, Jones J, Lister C: Behaviour of the maize transposable element Ac in Arabidopsis thaliana. Plant J 1: 69–81 (1992).Google Scholar
- 3.Haldane JBS, Waddington CH: Inbreeding and linkage. Genetics 16: 357–374 (1931).Google Scholar
- 6.Kosambi DD: The estimation of map distance from recombination values. Ann Eugen 12: 172–175 (1944).Google Scholar