Euphytica

, Volume 53, Issue 3, pp 225–233 | Cite as

Genetics of isozyme variants in Populus tremula, P. tremuloides and their hybrids

  • L. A. Gallo
  • Th. Geburek
Article
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Summary

Eight Populus tremula and six P. tremuloides clones as well as 49 full-sib families were studied in GOT, LAP, 6-PGDH, and SKDH by horizontal starch gel electrophoresis. For GOT one polymorphic zone was found and segregation of seven full-sib families suggests Mendelian inheritance. For LAP and 6-PGDH two zones each were clearly scored. For LAP two polymorphic loci were proposed based on the phenotypic segregation of isozyme variants in six and 34 full-sib families, respectively. In 24 full-sibs families the presence of ‘null alleles’ was inferred for both loci. The genetic control of the upper zone of 6-PGDH was demonstrated by a segregation analysis of 17 full-sib families. SKDH also demonstrated a Mendelian inheritance pattern in 12 of the full-sib families analysed. The electrophoretic patterns of pollen were similar to those of buds, but migration rates of the supposed corresponding isozymes were slightly modified (Lap-B, Skdh, 6Pgdh-A). Lap-A was not present in pollen extracts and hybrid bands were not found when gels were stained for dimeric enzymes (6-PGDH, GOT).

Key words

Populus tremula Populus tremuloides poplar isozymes genetic control null alleles pollen gene expression 
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References

  1. Allendorf, F.W., K.L. Knudsen & G.M. Blake, 1982. Frequencies of null alleles at enzyme loci in natural populations of ponderosa pine and red pine. Genetics 100: 497–504.Google Scholar
  2. Bergmann, F., 1981. Unterscheidung von Pappelklonen mit Hilfe von Isoenzym-Mustern. Die Holzzucht 35: 24–27.Google Scholar
  3. Bergmann, F. 1987. Characterization of multiclonal aspen cultivars using isozyme electrophoresis. For. Ecol. Manage. 22: 167–172.Google Scholar
  4. Cheliak, W.M. & B.P. Dancik, 1982. Genic diversity of natural populations of a clone-forming tree Populus tremuloides. Can. J. Genet. Cytol. 24: 611–616.Google Scholar
  5. Cheliak, W.M. & J.A. Pitel, 1984a. Electrophoretic identification of clones in trembling aspen. Can. J. For. Res. 14: 740–743.Google Scholar
  6. Cheliak, W.M. & J.A. Pitel, 1984b. Techniques for starch gel electrophoresis of enzymes from forest tree species. Information Report PI-X-42, Petawawa National Forestry Institute.Google Scholar
  7. Farmer Jr., R.E., W.M. Cheliak & J.A. Pitel, 1988. Isozyme variation in balsam poplar along a latitudinal transect in northwestern Ontario. Can. J. For. Res. 18: 1078–1081.Google Scholar
  8. Fieldes, M.A. & T.J. Gray, 1988. Rm differences in leaf malate dehydrogenases of flax (Linum usitatissimum) genotrophs: Apparent developmental effects. Journal of Experimental Botany 39: 499–509.Google Scholar
  9. Gallo, L.A., B.R. Stephan & D. Krusche, 1985. Genetic variation of Melampsora leaf rust resistance in progenies of crossings between and within Populus tremula and P. tremuloides clones. Silvae Genetica 34: 208–214.Google Scholar
  10. Gillet, E. & H.H. Hattemer, 1989. Genetic analysis of isozyme phenotypes using single tree progenies. Heredity 63: 135–141.Google Scholar
  11. Guzina, V., 1978. Genetic control of isoperoxidases Px-A1, Px-A2, Px-b1 and Px-B2 in aspen (Populus tremula L.). Ann. Forestales 8: 51–90.Google Scholar
  12. Harris, H. & D.A. Hopkins, 1976. Handbook of Enzyme Electrophoresis in Human Genetics North Holland Publ. Comp., Amsterdam, Oxford.Google Scholar
  13. Herrmann, S., 1969. Practical method to conserve pollen of forest trees under vacuum. 2nd. World Consult. on Forest Tree Breeding, Washington, FAO/IUFRO, FO-FTB-69–11/10: p. 1381–1389.Google Scholar
  14. Hyun, J.O., 1984. Inheritance of isozymes in root tips of trembling aspen (Populus tremuloides Michx.). J. Korean For. Soc. 64: 20–25.Google Scholar
  15. Hyun, J.O., O.P. Rajora & L. Zsuffa, 1987. Inheritance and linkage of isozymes in Populus tremuloides (Michx.). Genome 29: 384–388.Google Scholar
  16. Kim, C.S. & S.B. Chung, 1974. Variation in the patterns of isoperoxidase in the genus Populus. I. Variation in the patterns of isoperoxidase in P. alba, P. glandulosa and P. euramericana. Research Report of the Institute of Forest Genetics, Korea, 11: 53–59.Google Scholar
  17. Kim, C.S. & S.S. Kim, 1977. Variation in the pattern of isoperoxidase in genus Populus—Patterns of isoperoxidase in leaves of 15 clones of Populus albaglandulosa. J. of Korean Forestry 36: 1–4.Google Scholar
  18. Marquard, R.D. & R.G. Skorpenske, 1989. Expression of heritable biochemical markers from various pecan tissues. Euphytica 42: 65–70.Google Scholar
  19. Mäkinen, Y. & T. Macdonald, 1968. Isoenzyme polymorphism in flowering plants II. Pollen enzynmes and isoenzymes. Physiologia Plantarum 21: 477–486.Google Scholar
  20. Mather, K., 1957. The Measurement of Linkage in Heredity. Methuen and Co., London.Google Scholar
  21. Mitton, J.B. & M.C. Grant, 1980. Observations on the ecology and evolution of quaking aspen, Populus tremuloides, in the Colorado Front Range. Amer. J. Bot. 67: 202–209.Google Scholar
  22. Nikolié, D. & N. Tućić, 1983. Clinal variation of null alleles in european black pine (Pinus nigra Arn.). Genetika 15: 105–113.Google Scholar
  23. Pedersen, S., 1988. Variation among pollen specific isozymes in barley. Hereditas 109: 239–244.Google Scholar
  24. Pitel, J.A. & W.M. Cheliak, 1984. Effects of extraction buffers on characterization of isoenzymes from vegetative tissues of five conifer species: a user's manual. Information Report PI-X-34, Petawawa National Forestry Institute.Google Scholar
  25. Poulik, M.D., 1957. Starch gel electrophoresis in a discontinuous system of buffers. Nature 275: 1477–1479.Google Scholar
  26. Rajora, O.P., 1986. Studies into genetics and species relationships of Populus deltoides Marsh., P. nigra L. and P. maximowiczii Henry based on isozymes, pollen competition and leaf morphology. Ph. D. thesis, University of Toronto.Google Scholar
  27. Rajora, O.P. & L. Zsuffa, 1986. Sporophytic and gametophytic gene expression in Populus deltoides Marsh., P. nigra L., and P. maximowiczii Henry. Can. J. Genet. Cytol. 28: 476–482.Google Scholar
  28. Strauss, S.H. & M.T. Conkle, 1986. Segregation, linkage, and diversity of allozymes in knobcone pine. Theor. Appl. Genet. 72: 483–493.CrossRefGoogle Scholar
  29. Voelker, R.A., C.H. Langley, A.J. Leigh Brown, S. Ohnishi, B. Dickson, E. Montgomery & S.C. Smith, 1980a. Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population. Proc. Natl. Acad. Sci. USA, Vol. 77, No. 2: 1091–1095.Google Scholar
  30. Voelker, R.A., H.E. Schaffer & T. Mukai, 1980b. Spontaneous allozyme mutations in Drosophila melanogaster: rate of occurrence and nature of the mutants. Genetics 94: 961–968.Google Scholar
  31. Weber, J.C. & R.F. Stettler, 1981. Isoenzyme variation among ten populations of Populus trichocarpa Torr. et Gray in the Pacific Northwest. Silvae Genetica 30: 2–3.Google Scholar
  32. Weeden, N.F. & L.B. Gottlieb, 1979. Distinguishing allozymes and isozymes of phosphoglucoisomerase by electrophoretic comparisons of pollen and somatic tissues. Biochem. Genetics 17: 287–296.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • L. A. Gallo
    • 2
  • Th. Geburek
    • 1
  1. 1.Institute of Forest Genetics and Forest Tree BreedingGrosshansdorf 2Germany
  2. 2.E.E.A. Alto Valle de Rio NegroInstituto Nacional de Tecnologia AgropecuariaGeneral RocaArgentina

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