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Euphytica

, Volume 9, Issue 1, pp 111–121 | Cite as

Cytogenetics of the trispecific hybrid Nicotiana tabacum × (N. glutinosa × N. trigonophylla) and its reciprocal

  • K. V. Krishnamurty
  • G. S. Murty
  • K. Appa Rao
Article
  • 34 Downloads

Abstract

The paper deals with the cytogenetics of a new three species hybrid combination obtained as a result of crossing N. tabacum with the fertile amphiploid N. glutinosa × N. trigonophylla. The amphidiploid was established through colchicine doubling of a sterile hybrid of the same parentage in 1956. The tabacum-amphidiploid cross was made reciprocally and meiosis of both the hybrids was studied. While the N. tabacum × (glutinosa-trigonophylla) hybrid showed a pairing frequency of 6.09 bivalents (2 n of hybrid: 48) the reciprocal (glutinosa-trigonophylla) × N. tabacum, showed a mean bivalent frequency of 10.07. Other stages of meiosis also revealed differences. Pollen fertility was reduced to less than 1.0% in both and the hybrids proved to be sterile although one of them, (glutinosa-trigonophylla) × N. tabacum, exhibited a heavy setting of parthenocarpic capsules. In spite of continued failures, germination could be induced in a single seed of a backcross between N. tabacum × (glutinosa-trigonophylla) and N. tabacum.

While the amphiploid of the original cross possessed predominance of glutinosa characters, the reciprocal hybrids showed a greater expression of tabacum characters.

The pairing behaviour of the hybrids is discussed in the light of pairing behaviour of the same species in other hybrid combinations and a tentative assumption is drawn explaining the affinity between N. trigonophylla and N. tomentosiformis (present in the tabacum genome), confirming, to a certain extent, the statement that a relationship appears to be evident between the progenitors of the sections tomentosae and trigonophyllae, both with 12 chromosomes.

Keywords

Colchicine Pollen Fertility Sterile Hybrid Hybrid Combination Species Hybrid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Samenvatting

Met behulp van colchicinebehandeling werd in 1956 de steriele bastaard van Nicotiana glutinosa x N. trigonophylla verdubbeld. De aldus verkregen fertiele amphidiploid (welke onvatbaar was voor het tabaksmozaïek-virus) werd reciprook gekruist met N. tabacum. De uit genoemde 3 soorten opgebouwde bastaarden bezitten 48 chromosomen. Ze zijn in hoge mate steriel. Slechts 1 kiemend zaad werd verkregen van de terugkruising met N. tabacum.

Terwijl de amphidiploiden van de oorspronkelijke kruising vooral glutinosa eigenschappen vertoonden, hadden de reciproke bastaarden met N. tabacum meer tabacum-kenmerken.

In het gedrag van chromosomen-paring werd een bevestiging gevonden van de verwantschap tussen de 12-chromosomige voorouders van de secties tomentosae en trigonophyllae.

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Copyright information

© H. Veenman En Zonen N.V. 1960

Authors and Affiliations

  • K. V. Krishnamurty
    • 1
  • G. S. Murty
    • 1
  • K. Appa Rao
    • 1
  1. 1.Central Tobacco Research InstituteRajahmundryIndia

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