Plant Molecular Biology

, Volume 25, Issue 2, pp 313–318 | Cite as

Molecular characterisation of cDNA clones representing pectin esterase isozymes from tomato

  • Lisa N. Hall
  • Colin R. Bird
  • Steve Picton
  • Gregory A. Tucker
  • Graham B. Seymour
  • Donald Grierson
Short Communications


Two pectin esterase cDNA clones representing different isozymes with ca. 95% homology were isolated from an early ripening tomato fruit cDNA library. Both clones were longer than previously published sequences, and the encoded proteins possessed extended (229–233 amino acid) putative N-terminal extensions. In addition, the mRNA species corresponding to the two clones showed differential levels of expression in fruit.

Key words

cDNA fruit pectin esterase tomato 


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  1. 1.
    Bird CR, Smith CJS, Ray JA, Moureau P, Bevan MW, Bird AS, Hughes S, Morris PC, Grierson D, Schuch W: The tomato polygalacturonase gene and ripening-specific expression in transgenic plants. Plant Mol Biol 11: 651–662 (1984).Google Scholar
  2. 2.
    Delincée H: Thin-layer focusing of multiple forms of pectinesterase. Phytochemistry 15: 903–906 (1976).CrossRefGoogle Scholar
  3. 3.
    Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W: Sequencing and identification of a cDNA clone for tomato polygalacturonase. Nucl Acids Res 14: 8595–8603 (1986).PubMedGoogle Scholar
  4. 4.
    Hall LN, Tucker GA, Smith CJS, Watson CF, Bundick Y, Boniwell JM, Fletcher JD, Ray JA, Schuch W, Bird CR, Grierson D: Antisense inhibition of pectin esterase gene expression in transgenic tomatoes. Plant J 3: 121–129 (1993).Google Scholar
  5. 4a.
    Harriman RW, Tieman DM, Handa AK: Molecular cloning of tomato pectin methylesterase gene and its expression in Rutgers, ripening inhibitor, nonripening, and Never ripe tomato fruits. Plant Physiol 97: 80–87 (1991).Google Scholar
  6. 5.
    Markovič O, Jörnvall H: Pectinesterase. The primary structure of the tomato enzyme. Eur J Biochem 158: 455–462 (1986).PubMedGoogle Scholar
  7. 6.
    Markovič O, Jörnvall H: Tomato and Aspergillus niger pectinesterases. Correlation of differences in reports: large species variations. Protein Seq Data Anal 3: 513–515 (1990).PubMedGoogle Scholar
  8. 7.
    Picton S, Gray J, Barton S, AbuBakar U, Lowe A, Grierson D: cDNA cloning and characterisation of novel ripening-related mRNAs with altered patterns of accumulation in the ripening inhibitor (rin) tomato ripening mutant. Plant Mol Biol, in press (1993).Google Scholar
  9. 8.
    Pressey R, Avants JK: Multiple forms of pectinesterase in tomatoes. Phytochemistry 11: 3139–3142 (1972).CrossRefGoogle Scholar
  10. 9.
    Pressey R, Avants JK: Solubilization of cell walls by tomato polygalacturonases: effects of pectinesterases. J Food Biochem 6: 57–74 (1982).Google Scholar
  11. 10.
    Ray J, Knapp J, Grierson D, Bird C, Schuch W: Identification and sequence determination of a cDNA clone for tomato pectin esterase. Eur J Biochem 174: 119–124 (1988).PubMedGoogle Scholar
  12. 11.
    Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbour, NY (1989).Google Scholar
  13. 12.
    Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).PubMedGoogle Scholar
  14. 13.
    Studencki AB, Wallace RB: Allele-specific hybridization using oligonucleotide probes of very high specific activity: discrimination of the human β A- and β B-globin genes. DNA 3: 7–15 (1984).PubMedGoogle Scholar
  15. 14.
    Tieman DH, Harriman RW, Ramamohan G, Handa AK: An antisense pectin methylesterase gene alters pectin chemistry and soluble solids in tomato fruit. Plant Cell 4: 667–679 (1992).CrossRefPubMedGoogle Scholar
  16. 15.
    Tucker GA, Robertson NG, Grierson D: Purification and changes in activity of tomato pectinesterase isoenzymes. J Sci Food Agric 33: 396–400 (1982).Google Scholar
  17. 16.
    von Heijne G: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683–4690 (1986).PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Lisa N. Hall
    • 1
  • Colin R. Bird
    • 2
  • Steve Picton
    • 1
  • Gregory A. Tucker
    • 3
  • Graham B. Seymour
    • 3
  • Donald Grierson
  1. 1.Department of Physiology and Environmental ScienceUniversity of NottinghamLoughboroughUK
  2. 2.Plant Biotechnology Section, ZENECA SeedsUK
  3. 3.Department of Applied Biochemistry and Food ScienceUniversity of NottinghamLoughboroughUK

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