Euphytica

, Volume 87, Issue 1, pp 59–64

Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica

  • P. Lashermes
  • P. Trouslot
  • F. Anthony
  • M. C. Combes
  • A. Charrier
Article

Summary

Random amplified polymorphic DNA (RAPD) markers have been successfully employed to analyse the genetic diversity among cultivated and subspontaneous accessions of Coffea arabica. The narrow genetic base of commercial cultivars was confirmed. On the other hand, a relatively large genetic diversity was observed within the germplasm collection demonstrating the importance of collecting missions. Results suggested an East-West differentiation in Ethiopia, the primary centre of diversification of C. arabica. The large heterosis effect reported in intergroup hybrids could be related to such genetic differentiation. RAPD method appeared to be effective in resolving genetic variations and in grouping germplasm in C. arabica.

Key words

Coffea arabica coffee diversity genetic resources heterosis RAPD 

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References

  1. Berthaud J. & A.Charrier, 1988. Genetics resources of Coffea. In: R.J.Clarke & R.Macrae (Eds) Coffee vol 4: Agronomy. Elsevier Applied Science, London, pp. 1–42.Google Scholar
  2. Berthaud J., J.L.Guillaumet, D.LePierres & M.Lourd, 1980. Les caféiers sauvages du Kenya: prospection et mise en culture. Café-Cacao-Thé 24: 101–112.Google Scholar
  3. Berthou, F. & P. Trouslot, 1977. L'analyse du polymorphisme enzymatique dans le genre Coffea: adaptation d'une méthode d'électrophorèse en série. 8th Conference of ASIC, Abidjan (Côte d'Ivore): 373–383.Google Scholar
  4. Bayetta-Bellachew, Mesfin-Ameha & Dejene-Mekonnen, 1993. Heterosis and combining ability in coffee (Coffea arabica L.). 15th Conference of ASIC, Montpellier (France): 234–242.Google Scholar
  5. Bouharmont P. & C. Montagnon, 1995. Diversité phenotypique de Coffea arabica observée en collection au Cameroun. 16th Conference of ASIC, Kyoto (Japan), in press.Google Scholar
  6. Carvalho A., 1988. Principles and practices of coffee plant breeding for productivity and quality factors: Coffea arabica. In: R.J.Clarke & R.Macrae (Eds) Coffee vol 4: Agronomy. Elsevier Applied Science, London, pp. 129–165.Google Scholar
  7. Carvalho A., H.P. MedinaFilho, L.C.Fazuoli, O. GuerreiroFilho & M.M.A.Lima, 1991. Aspectos genéticos do cafeeiro. Rev. Brasil. Genet. 14: 135–183.Google Scholar
  8. Charrier, A., 1978. Synthèse de neuf années d'observation et d'expérimentation sur les Coffea arabica collectés en Ethiopie par une mission ORSTOM. In: A. Charrier (Ed.) Etude de la structure et de la variabilité génétique des caféiers, IFCC (Paris), Bulletin no 14, pp. 4–10.Google Scholar
  9. Darlu P. & P.Tassy, 1993. Reconstruction phylogénétique: concepts et méthodes. Collection biologie théorique, Masson, Paris (France), 245 p.Google Scholar
  10. F.A.O., 1968. FAO coffee mission to Ethiopia 1964–1965. Report, FAO ed., Rome (Italy), 200 p.Google Scholar
  11. Guillaumet, J.L. & F. Hallé, 1978. Echantillonnage du materiel Coffea arabica récolté en Ethiopie. In: A. Charrier (Ed.) Etude de la structure et de la variabilité génétique des caféiers, IFCC (Paris), Bulletin no. 14, pp. 13–18.Google Scholar
  12. Krug C.A., J.E.T.Mendes & A.Carvalho, 1939. Taxonomia de Coffea arabica L. Descriçao das variedase formas encontradas no estado de Sao Paulo. Boletim Tecnico 62, Instituto Agronomico do Estado (Campinas, Brazil) 57 pGoogle Scholar
  13. Kruskal J.B., 1964a. Nonmetric multidimensional scaling: a numerical method. Psychometrika 29: 1–27.Google Scholar
  14. Kruskal J.B., 1964b. Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrika 29: 115–129.Google Scholar
  15. Lashermes P., J.Cros, P.Marmey & A.Charrier, 1993. Use of random amplified DNA markers to analyze genetic variability and relationships of Coffea species. Genetic Resources and Crop Evolution 40: 91–99.Google Scholar
  16. Lashermes, P., M.C. Combes, J. Cros, P. Trouslot, F. Anthony & A. Charrier, 1995. Origin and genetic diversity of Coffea arabica L. based on DNA molecular markers. 16th Conference of ASIC, Kyoto (Japan), in press.Google Scholar
  17. Meyer, G.G., 1969. The origin of arabica coffee. Proc. XI intl. bot. Congr., Seattle (USA), 146 p.Google Scholar
  18. Orozco-Castillo C., K.J.Chalmers, R.Waugh & W.Powell, 1994. Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87: 934–940.Google Scholar
  19. Shepard R.N., 1973. Introduction to vol. 1. In: R.N.Shepard, A.K.Romney & S.B.Nerlove (Eds) Multidimensional scaling, vol. 1, Seminar press, New York (USA).Google Scholar
  20. Smith F.R., 1985. A history of coffee. In: M.N.Clifford & K.C.Wilson (Eds) Coffee, Botany, Biochemistry and production of beans and beverage. Croom Helm, London & Sydney, pp. 1–12.Google Scholar
  21. Sylvain P.G., 1958. Ethiopian Coffee — Its significance to world coffee problems. Botany 12: 111–139.Google Scholar
  22. Van derVossen H.A.M., 1985. Coffee selection and breeding. In: M.N.Clifford & K.C.Wilson (Eds) Coffee, Botany, Biochemistry and production of beans and beverage. Croom Helm, London & Sydney, pp. 48–97.Google Scholar
  23. Walyaro, D.J.A., 1983. Considerations in breeding for improved yield and quality in arabica coffee (Coffea arabica L.). Doctoral thesis, Wageningen, The Netherlands, 122 p.Google Scholar
  24. Welsh J. & M.McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213–7218.Google Scholar
  25. Williams J.G.K., A.R.Kubelik, K.J.Livak, J.A.Rafalski & S.V.Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • P. Lashermes
    • 1
  • P. Trouslot
    • 1
  • F. Anthony
    • 2
  • M. C. Combes
    • 1
  • A. Charrier
    • 1
  1. 1.ORSTOMMontpellierFrance
  2. 2.CATIETurrialbaCosta Rica

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