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Euphytica

, Volume 79, Issue 3, pp 175–179 | Cite as

A genomic library of Lycopersicon pennellii in L. esculentum: A tool for fine mapping of genes

  • Yuval Eshed
  • Dani Zamir
Article

Abstract

The cultivated tomato contains only a small fraction of the genetic variation present in its wild relatives. In order to use the wild germplasm in tomato breeding and genetic studies we developed a new kind of genetic resource which is composed of 50 L. esculentum lines each containing a single introgression from the green fruited species L. pennellii (LA 716). Each of the introgression lines is nearly isogenic to the cultivated tomato; these lines provide complete coverage of the wild species genome. The lines contain on the average an introgression of 33 cM from a total genome size of 1200 cM. The size and identity of the introgressed segments was determined based on RFLP analysis of 350 markers. This resource can be viewed as a genomic library of the wild species in the cultivated background. It covers the entire genome with single independent ‘inserts’ per line and therefore every phenotypic difference between the introgression lines can be associated with the unique introgressed segment. The development and potential application of this resource are discussed.

Key words

Gene mapping genomic library Lycopersicon RFLP wild germplasm 

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References

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Yuval Eshed
    • 1
  • Dani Zamir
    • 1
  1. 1.Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for BiotechnologyThe Hebrew University of JerusalemRehovotIsrael

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