Plant Molecular Biology

, Volume 31, Issue 2, pp 443–448

Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2

  • Mark D. Burow
  • Zhi-Yuan Chen
  • Tricia M. Mouton
  • James V. Moroney
Short Communication

Abstract

Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.

Key words

carbonic anhydrase Chlamydomonas CO2 concentrating mechanism gene regulation by CO2 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Mark D. Burow
    • 1
  • Zhi-Yuan Chen
    • 1
  • Tricia M. Mouton
    • 1
  • James V. Moroney
    • 1
  1. 1.Department of Plant BiologyLouisiana State UniversityBaton RougeUSA
  2. 2.Department of Soil and Crop SciencesTexas A&M UniversityCollege StationUSA

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