Plant Molecular Biology

, Volume 31, Issue 2, pp 405–412 | Cite as

Characterization of eight new members of the calmodulin-like domain protein kinase gene family from Arabidopsis thaliana

  • Estelle M. Hrabak
  • Leslie J. Dickmann
  • John S. Satterlee
  • Michael R. Sussman
Short Communication


A family of calcium-responsive protein kinases is abundant in plant cell extracts but has not been identified in animals and fungi. These enzymes have a unique structure consisting of a protein kinase catalytic domain fused to carboxy-terminal autoregulatory and calmodulin-like domains. In this report, we present the amino acid sequences for eight new Arabidopsis cDNA clones encoding isoforms of this enzyme. Three isoforms were expressed as fusion proteins in Escherichia coli and exhibited calcium-stimulated protein kinase activity. We propose CPK as the gene designation for this family of enzymes and describe a phylogenetic analysis for all known isoforms.

Key words

Arabidopsis calcium calmodulin gene family kinase phylogeny 


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Estelle M. Hrabak
    • 1
  • Leslie J. Dickmann
    • 2
  • John S. Satterlee
    • 2
  • Michael R. Sussman
    • 2
  1. 1.Department of Plant BiologyUniversity of New HampshireDurhamUSA
  2. 2.Department of Horticulture and Program in Cellular and Molecular BiologyUniversity of WisconsinMadisonUSA

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