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Plant Molecular Biology

, Volume 23, Issue 4, pp 905–909 | Cite as

A conjugative plasmid vector for promoter analysis in several cyanobacteria of the genera Synechococcus and Synechocystis

  • Pierre Marraccini
  • Stéphane Bulteau
  • Corinne Cassier-Chauvat
  • Pierre Mermet-Bouvier
  • Franck Chauvat
Short Communication

Abstract

A promoter-probe vector, pSB2A, based on the plasmid RSF1010 and the promoterless chloramphenicol acetyl transferase (cat) reporter gene, has been constructed. pSB2A appeared to be most efficiently transferred by conjugation to the widely used cyanobacteria Synechocystis strains PCC6803 (S.6803) and PCC6714 (S.6714) and Synechococcus strains PCC7942 (S.7942) and PCC6301 (S.6301), where it replicates stably even though it contains no cyanobacterial DNA. Using pSB2A we found that (1) a light-regulated promoter from S.6803 remains controlled by light intensity in S.7942 while it is silent in Escherichia coli, and (2) the E. coli tac promoter behaves as a strong and light-independent promoter in the four cyanobacterial hosts tested.

Key words

conjugation cyanobacteria light regulation promoter probe RSF1010 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Pierre Marraccini
  • Stéphane Bulteau
  • Corinne Cassier-Chauvat
    • 1
  • Pierre Mermet-Bouvier
  • Franck Chauvat
  1. 1.CNRS, URA D1290, Départment de Biologie Cellulaire et Moléculaire, Commissariat à l'Energie Atomiqu eCentre d'Etudes de SaclayGif-sur-Yvette CedexFrance

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