Plant Molecular Biology

, Volume 23, Issue 1, pp 57–66 | Cite as

Two members of the ERabp gene family are expressed differentially in reproductive organs but to similar levels in the coleoptile of maize

  • Thomas Hesse
  • Christine Garbers
  • Bretislav Brzobohaty
  • Georg Kreimer
  • Dieter Söll
  • Michael Melkonian
  • Jeff Schell
  • Klaus Palme
Research Article


A Zea mays cDNA clone, ZmERabp4, coding for a new member of the auxin-binding protein family was isolated. The primary amino acid sequence contains an N-terminal hydrophobic leader sequence, a potential glycosylation site (Asn136-Thr-Thr) and a C-terminal KDEL motif known to be responsible for retention of proteins within the lumen of the ER. The expression pattern of the ZmERabp4 gene in various organs of maize differs from the expression pattern previously observed for the ZmERabp1 gene. The ZmERabp4 gene is expressed highly in male flower organs, whereas the ZmERabp1 gene shows highest expression in female flower parts. In situ hybridization and analysis by laser scanning microscopy revealed enhanced levels of expression for both genes in the coleoptile when compared with the primary leaf of etiolated maize seedlings.

Key words

auxin auxin-binding protein auxin receptor ERabp gene differential expression maize (Zea mays L.) in situ hybridization confocal laser scanning microscopy 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Thomas Hesse
    • 1
  • Christine Garbers
    • 1
  • Bretislav Brzobohaty
    • 1
  • Georg Kreimer
    • 2
  • Dieter Söll
    • 3
  • Michael Melkonian
    • 2
  • Jeff Schell
    • 1
  • Klaus Palme
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKölnGermany
  2. 2.Botanisches InstitutLehrstuhl I der Universität zu KölnKölnGermany
  3. 3.Department of Molecular Biophysics and BiochemistryYale UniversityNew HavenUSA

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