Plant Molecular Biology

, Volume 8, Issue 4, pp 291–298

Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens

  • Shahla N. Sheikholeslam
  • Donald P. Weeks
Article

Abstract

High frequency transformation of Arabidopsis thaliana leaf explants has been obtained using a disarmed Ti plasmid containing the coding region of a neomycin phosphotransferase gene (NPT II) as a selectable marker. The rate of transformation ranged from 55 to 63 percent when acetosyringone (AS), a natural wound response molecule, was added to an Agrobacterium tumefaciens culture prior to incubation with leaf segments. Without acetosyringone, the transformation rate was approximately 2 to 3 percent. Calli resistant to G418 were regenerated into mature flowering plants in the presence of 10 μg/ml G418. Southern analysis and neomycin phosphotransferase assays confirmed the insertion and expression of the NPT II gene in regenerated Arabidopsis plants.

Keywords

acetosyringone Agrobacterium tumefaciens Arabidopsis thaliana leaf explants regeneration transformation 

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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Shahla N. Sheikholeslam
    • 1
  • Donald P. Weeks
    • 1
  1. 1.Molecular Biology Department, Zoecon Research InstituteSandoz Crop Protection CorporationPalo AltoUSA

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