Plant Molecular Biology

, Volume 8, Issue 4, pp 291–298

Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens

  • Shahla N. Sheikholeslam
  • Donald P. Weeks
Article

DOI: 10.1007/BF00021308

Cite this article as:
Sheikholeslam, S.N. & Weeks, D.P. Plant Mol Biol (1987) 8: 291. doi:10.1007/BF00021308

Abstract

High frequency transformation of Arabidopsis thaliana leaf explants has been obtained using a disarmed Ti plasmid containing the coding region of a neomycin phosphotransferase gene (NPT II) as a selectable marker. The rate of transformation ranged from 55 to 63 percent when acetosyringone (AS), a natural wound response molecule, was added to an Agrobacterium tumefaciens culture prior to incubation with leaf segments. Without acetosyringone, the transformation rate was approximately 2 to 3 percent. Calli resistant to G418 were regenerated into mature flowering plants in the presence of 10 μg/ml G418. Southern analysis and neomycin phosphotransferase assays confirmed the insertion and expression of the NPT II gene in regenerated Arabidopsis plants.

Keywords

acetosyringone Agrobacterium tumefaciens Arabidopsis thaliana leaf explants regeneration transformation 

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Shahla N. Sheikholeslam
    • 1
  • Donald P. Weeks
    • 1
  1. 1.Molecular Biology Department, Zoecon Research InstituteSandoz Crop Protection CorporationPalo AltoUSA

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