Plant Molecular Biology

, Volume 27, Issue 6, pp 1197–1204

Structure and induction pattern of a novel proteinase inhibitor class II gene of tobacco

  • Teresa Balandin
  • Chris van der Does
  • José-Maria Bellés Albert
  • John F. Bol
  • Huub J. M. Linthorst
Short Communication

Abstract

A cDNA and a corresponding genomic clone encoding a protein with partial identity to type II proteinase inhibitors from potato, tomato and Nicotiana alata, were isolated from tobacco libraries. The protein of 197 amino acids contains a putative signal peptide of 24 residues and three homologous domains, each with a different reactive site. The tobacco PI-II gene is not expressed in leaves of healthy plants, but is locally induced in leaves subjected to different types of stress (TMV infection, wounding, UV irradiation) and upon ethephon treatment. As opposed to the analogous PI-II genes of potato and tomato, the tobacco gene is not systemically induced by wounding or pathogenic infection. A far-upstream region in the PI-II promoter, containing various direct and indirect repeats, shares considerable sequence similarity to a similar region in the stress-inducible Cu/Zn-superoxide dismutase gene of N. plumbaginifolia.

Key words

pathogenesis-related proteins proteinase inhibitor signal transduction wounding 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Teresa Balandin
    • 1
  • Chris van der Does
    • 1
  • José-Maria Bellés Albert
    • 1
  • John F. Bol
    • 1
  • Huub J. M. Linthorst
    • 1
  1. 1.Institute of Molecular Plant SciencesGorlaeus LaboratoriesLeidenThe Netherlands
  2. 2.Department of Molecular MicrobiologyGroningen UniversityHarenThe Netherlands
  3. 3.Dep. Biotecnología, E.T.S.I. AgrónomosUniv. PolitécnicaValenciaSpain

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