Plant Molecular Biology

, Volume 30, Issue 5, pp 1067–1073 | Cite as

Comparison of the expression patterns of genes coding for wheat gluten proteins and proteins involved in the secretory pathway in developing caryopses of wheat

  • Barbara Grimwade
  • Arthur S. Tatham
  • Robert B. Freedman
  • Peter R. Shewry
  • Johnathan A. Napier
Short Communication


The synthesis of gluten proteins in the developing caryopsis of wheat is highly coordinated, with mRNAs for the various groups being detected from 11 days after anthesis, and the proteins from about 14 days. In contrast, the levels of transcripts for BiP, PDI and PPI are highest at earlier stages of development. The levels of transcripts for two small GTP binding proteins involved in the secretory pathway (Rab1 and Rab5) are also highest early in development, which is consistent with the retention of most of the gluten proteins within the ER to form protein bodies.

Key words

seed development molecular chaperones small GTP-binding proteins storage protein synthesis 


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  1. 1.
    Altschuler Y, Rosenburg N, Harel R, Galili G: The N-and C-terminal regions regulate the transport of wheat γ-gliadin through the endoplasmic reticulum in Xenopus oocytes. Plant Cell 5: 443–450 (1993).CrossRefPubMedGoogle Scholar
  2. 2.
    Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M: Nucleotide sequence of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv. Cheyenne. Nucl Acids Res 17: 461–462 (1989).PubMedGoogle Scholar
  3. 3.
    Boston RS, Fontes EBP, Shank BB, Wrobel RL: Increased expression of the maize immunoglobulin binding protein homolog b-70 in three zein regulatory mutants. Plant Cell 3: 497–505 (1991).CrossRefPubMedGoogle Scholar
  4. 4.
    Brandt A: Endosperm protein formation during kernel development of wild type and a high-lysine barley mutant. Cereal Chem 53: 890–894 (1976).Google Scholar
  5. 5.
    Bulleid NJ, Freedman RB: Defective co-translational formation of disulphide bonds in protein disulphide isomerase deficient microsomes. Nature 335: 649–651 (1988).CrossRefPubMedGoogle Scholar
  6. 6.
    Colot V, Bartels D, Thompson R, Flavell RB: Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes. Mol Gen Genet 216: 81–90 (1989).CrossRefPubMedGoogle Scholar
  7. 7.
    Cox JS, Shamu CE, Walter P: Transcriptional induction of genes encoding reticulum resident proteins requires a transmembrane protein kinase. Cell 73: 1197–1206 (1993).CrossRefPubMedGoogle Scholar
  8. 8.
    Galterio G, Biancolatte E, Autran JC: Proteins deposition in developing durum wheat. Implications in technological quality. Genet Agric 41: 461–480 (1987).Google Scholar
  9. 9.
    Giorini S, Galili G: Characterization of HSP-70 cognate proteins from wheat. Theor Appl Genet 82: 615–620 (1991).CrossRefGoogle Scholar
  10. 10.
    Greene FC, Anderson OD, Litts JC, Gautier M-F: Control of wheat protein biosynthesis. Cereal Chem 62: 398–405 (1985).Google Scholar
  11. 11.
    Hull GA, Halford NG, Kreis M, Shewry PR: Isolation and characterisation of genes encoding rye prolamins containing a highly repetitive sequence motif. Plant Mol Biol 17: 1111–1115 (1991).PubMedGoogle Scholar
  12. 12.
    Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).PubMedGoogle Scholar
  13. 13.
    Levanony H, Rubin R, Altschuler Y, Galili G: Evidence for a novel route of wheat storage proteins to vacuoles, J Cell Biol 119: 1117–1128 (1992).CrossRefPubMedGoogle Scholar
  14. 14.
    Li X, Wu Y, Zhang D-Z, Gillikin JW, Boston RS, Franceschi VR, Okita TW: Rice prolamine protein body biogenesis: A BiP-mediated process. Science 262: 1054–1056 (1993).PubMedGoogle Scholar
  15. 15.
    Munro S, Pelham HRB: An hsp 70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46: 291–300 (1986).CrossRefPubMedGoogle Scholar
  16. 16.
    Napier JA, Smith MA, Stobart AK, Shewry PR: Isolation of a cDNA encoding a cytochrome b5 specifically expressed in developing tobacco seeds. Planta 197: 1200–202 (1995).CrossRefGoogle Scholar
  17. 17.
    Ng PKW, Slominski E, Johnson WJ, Bushuk W: Changes in wheat endosperm proteins during grain maturation. In: Bushuk W, Tkachuk R (eds) Gluten Proteins, pp. 740–754. American Association of Cereal Chemists, St. Paul, MN (1990).Google Scholar
  18. 18.
    Parker ML: Protein body inclusions in developing wheat endosperm. Ann Bot 46: 29–36 (1980).Google Scholar
  19. 19.
    Rahman S, Kreis M, Forde BG, Shewry PR, Miflin BJ: Hordein-gene expression during development of the barley (Hordeum vulgare) endosperm. Biochem J 223: 315–322 (1984).PubMedGoogle Scholar
  20. 20.
    Reeves CD, Krishnan HB, Okita TW: Gene expression in developing wheat endosperm. Plant Physiol 82: 34–40 (1986).Google Scholar
  21. 21.
    Roden LT, Miflin BJ, Freedman RB: Protein-disulphide isomerase is located in the endoplasmic reticulum of developing wheat endosperm. FEBS Lett 138: 121–124 (1982).CrossRefGoogle Scholar
  22. 22.
    Rosenberg N, Shimoni Y, Altschuler Y, Levanony H, Volokita M, Galili G: Wheat (Triticum aestivum L.) γ-gliadin accumulates in dense protein bodies within the endoplasmic reticulum of yeast. Plant Physiol 102: 61–69 (1993).PubMedGoogle Scholar
  23. 23.
    Rubin R, Levanony H, Galili G: Evidence for the pressence of two different types of protein bodies in wheat endosperm. Plant Physiol 99: 718–724 (1992).Google Scholar
  24. 24.
    Sabelli PA, Shewry PR: Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis. Theor Appl Genet 83: 209–216 (1991).CrossRefGoogle Scholar
  25. 25.
    Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).Google Scholar
  26. 26.
    Seilmeier W, Wieser H, Belitz H-D: Weizen wahrend der Reifung: Analyse der Gliandine und Glutenine mittels RP-HPLC. Z LebensmUnters Forsch 191: 99–103 (1990).Google Scholar
  27. 27.
    Shwery PR, Miles MJ, Tatham AS: The prolamin storage proteins of wheat and related species. Prog Biophys Mol Biol 61: 37–59 (1993).Google Scholar
  28. 28.
    Shewry PR, Napier JA, Tatham AS: Seed storage proteins: structures and biosynthesis. Plant Cell 7: 945–956 (1995).CrossRefPubMedGoogle Scholar
  29. 29.
    Shewry PR, Pratt HM, Leggatt MM, Miflin BJ: Protein metabolism in developing endosperms of high-lysine and normal barley. Cereal Chem 56: 100–117 (1979).Google Scholar
  30. 30.
    Shimoni Y, Zhu X-Z, Levanony H, Segal G, Galili G: Purification, characterization, and intracellular localization of glycosylated protein disulphide isomerase from wheat grains. Plant Physiol 108: 327–335 (1995).CrossRefPubMedGoogle Scholar
  31. 31.
    Smith MA, Stobart AK, Shewry PR, Napier JA: Tobacco cytochrome b 5: cDNA isolation, expression analysis and in vitro protein targeting. Plant Mol Biol 25: 527–537 (1994).PubMedGoogle Scholar
  32. 32.
    Stamnes MA, Rutherford SL, Zuker CS: Cyclophilins: a new family of proteins involved in intracellular folding. Trends Cell Biol 2: 272–276 (1992).CrossRefPubMedGoogle Scholar
  33. 33.
    Tamas L, Greenfield J, Halford NG, Tatham AS, Shewry PR: A β-turn rich barley seed protein in correctly folded in E. coli. Prot Express Purific 5: 357–363 (1994).CrossRefGoogle Scholar
  34. 34.
    Tatham AS, Shewry PR, Belton PS: 13C NMR study of C hordein. Biochem J 232: 617–620 (1985).PubMedGoogle Scholar
  35. 35.
    Terryn N, Van Montagu M, Inze D: GTP-binding proteins in plants. Plant Mol Biol 22: 143–152 (1993).PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Barbara Grimwade
    • 1
    • 2
  • Arthur S. Tatham
    • 1
  • Robert B. Freedman
    • 2
  • Peter R. Shewry
    • 1
  • Johnathan A. Napier
    • 1
  1. 1.IACR-Long Ashton Research Station, Department of Agricultural SciencesUniversity of BristolBristolUK
  2. 2.Biological LaboratoryUniversity of Kent at CanterburyCanterburyUK

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