Plant Molecular Biology

, Volume 30, Issue 5, pp 973–982 | Cite as

Characterization of a cDNA encoding a proline-rich 14 kDa protein in developing cortical cells of the roots of bean (Phaseolus vulgaris) seedlings

  • Dong-Woog Choi
  • Jai Young Song
  • Young Myung Kwon
  • Sang-Gu Kim
Research Article

Abstract

A cDNA clone, corresponding to mRNAs preferentially expressed in the roots of bean (Phaseolus vulgaris L.) seedlings, was isolated. This clone contains a 381 bp open reading frame encoding a polypeptide of 13.5 kDa, designated PVR5 (Phaseolus vulgaris root 5). The amino acid sequence of this clone is rich in proline (13.5%) and leucine (12.7%) and shares significant amino acid sequence homology with root-specific and proline-rich proteins from monocots (maize and rice), and proline-rich proteins from dicots (carrot, oilseed rape, and Madagascar periwinkle). The precise biological roles of these polypeptides are unknown. PVR5 mRNA accumulation is developmentally regulated within the root, with high levels at the root apex and declining levels at distances further from the root tip. In situ hybridization shows that PVR5 mRNA specifically accumulates in the cortical ground meristem in which maximal cell division occurs. Southern blot analysis suggests that genomic DNA corresponding to PVR5 cDNA is encoded by a single gene or a small gene family.

Key words

cysteine-rich domain 14 kDa proline-rich protein root-specific gene Phaseolus vulgaris 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Dong-Woog Choi
    • 1
  • Jai Young Song
    • 1
  • Young Myung Kwon
    • 1
  • Sang-Gu Kim
    • 1
  1. 1.Department of Biology and Research Center for Cell DifferentiationSeoul National UniversitySeoulKorea

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