Plant Molecular Biology

, Volume 30, Issue 5, pp 863–872

Expression analysis of a sucrose synthase gene from sugar beet (Beta vulgaris L.)

  • Holger Hesse
  • Lothar Willmitzer
Research Article

DOI: 10.1007/BF00020799

Cite this article as:
Hesse, H. & Willmitzer, L. Plant Mol Biol (1996) 30: 863. doi:10.1007/BF00020799

Abstract

To investigate the expression pattern of sucrose synthase, a cDNA from tap roots of sugar beet (Beta vulgaris L.) was isolated using a heterologous sucrose synthase cDNA from potato. The 2762 bp long cDNA clone designated SBSS 1 encodes for a 822 amino acid polypeptide of a predicted molecular mass of 93.7 kDa. The deduced amino acid sequence of sugar beet sucrose synthase has homologies of 65–70% when compared to predicted amino acid sequences of sucrose synthases from other species. RNA blot analysis shows that SBSS1 is expressed most predominant in tap root under normal growth conditions. Cold treatment and anaerobiosis lead to an increase in the steady-state levels of SBSS 1 mRNA in leaf and root tissue. In tap root slices, sugars in various concentrations had no influence on the SBSS 1 transcript level. On the other hand, wounding resulted in a decreased transcript level.

Key words

gene expression sucrose synthase sugar beet (Beta vulgaris L.) 

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Holger Hesse
    • 1
  • Lothar Willmitzer
    • 1
  1. 1.Institut für Genbiologische Forschung Berlin GmbHBerlinGermany

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