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Plant Molecular Biology

, Volume 5, Issue 5, pp 299–302 | Cite as

A chimaeric hygromycin resistance gene as a selectable marker in plant cells

  • Peter J. M. van den Elzen
  • Jeffrey Townsend
  • Kathleen Y. Lee
  • John R. Bedbrook
Short Communication

Summary

We show here that plant cells are sensitive to the antibiotic hygromycin-B4. We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments.

Keywords

Ti plasmid Hygromycin transformation 

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Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • Peter J. M. van den Elzen
    • 1
  • Jeffrey Townsend
    • 1
  • Kathleen Y. Lee
    • 1
  • John R. Bedbrook
    • 1
  1. 1.Advanced Genetic Sciences, Inc.OaklandU.S.A.

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