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Plant Molecular Biology

, Volume 30, Issue 2, pp 373–379 | Cite as

Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds

  • Chae Oh Lim
  • Soo In Lee
  • Woo Sik Chung
  • Sung Han Park
  • Inhwan Hwang
  • Moo Je Cho
Short communication

Abstract

A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.

Key words

Chinese cabbage (Brassica campestris L. spp. pekinensiscDNA cystatin inhibitor gene expression 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Chae Oh Lim
    • 1
    • 2
  • Soo In Lee
    • 1
    • 2
  • Woo Sik Chung
    • 1
    • 2
  • Sung Han Park
    • 1
    • 2
  • Inhwan Hwang
    • 1
    • 2
  • Moo Je Cho
    • 1
    • 2
  1. 1.Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityChinjuKorea
  2. 2.Department of BiochemistryGyeongsang National UniversityChinjuKorea

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