Plant Molecular Biology

, Volume 18, Issue 4, pp 827–833

Sequence analysis of linked maize 22 kDa α-zein genes

  • Gary A. Thompson
  • David R. Siemieniak
  • Leang C. Sieu
  • Jerry L. Slightom
  • Brian A. Larkins
Update Section Short Communication


We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa α-zein genes. The 5′ gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5′ and 3′ flanking regions that are typical of zein genes. In contrast, the 3′ gene (ψgZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5′ and 3′ flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.

Key words

α-zein gene duplication maize genes maize pseudogene 


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  1. 1.
    Argos P, Pederson K, Marks MD, Larkins BA: A structural model for maize zein proteins. J Biol Chem 257: 9984–9990 (1982).PubMedGoogle Scholar
  2. 2.
    Boronat A, Martinez MC, Reina M, Puigdomenech P, Palau J: Isolation and sequencing of a 28 kD glutelin-2 gene from maize. Common elements in the 5′ flanking regions among zein and glutelin genes. Plant Sci 47: 95–102 (1986).CrossRefGoogle Scholar
  3. 3.
    Bosma PJ, van denBerg EA, Kooistra T, Siemieniak DR, Slightom JL: Human plasminogen activator inhibitor-1 gene. Promoter and structural gene nucleotide sequences. J Biol Chem 263: 9129–9141 (1988).PubMedGoogle Scholar
  4. 4.
    Calzone FJ, Britten RJ, Davidson EH: Mapping of gene transcripts by nuclease protection assays and cDNA primer extension. In: Berger SL, Kimmel AR (eds) Methods in Enzymology: Guide to Molecular Cloning Techniques, pp. 611–632. Academic Press, New York (1987).Google Scholar
  5. 5.
    Elliston K, Messing J: The molecular architecture of plant genes and their regulation. In: Kung S, Arntzen CJ (eds) Plant Biotechnology, pp. 115–139. Butterworths, Boston (1989).Google Scholar
  6. 6.
    Esen A: Separation of alcohol-soluble proteins (zeins) from maize into three fractions by differential solubility. Plant Physiol 80: 623–627 (1986).Google Scholar
  7. 7.
    Gianazza E, Viglienghi V, Righetti PC, Salamini F, Soave C: Amino acid composition of zein molecular component. Phytochemistry 16: 315–317 (1977).CrossRefGoogle Scholar
  8. 8.
    Hagen G, Rubenstein I: Complex organization of zein genes in maize. Gene 13: 239–249 (1981).PubMedGoogle Scholar
  9. 9.
    Heidecker G, Messing J: Structural analysis of plant genes. Annu Rev Plant Physiol 37: 439–466 (1986).Google Scholar
  10. 10.
    Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643–6653 (1987).PubMedGoogle Scholar
  11. 11.
    Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ: Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley, and rye. In: Miflin BJ (ed) Oxford Surveys of Plant Molecular and Cell Biology, vol 2, pp. 253–317. Oxford University Press, Oxford (1985).Google Scholar
  12. 12.
    Kridl JC, Vieira J, Rubenstein I, Messing J: Nucleotide sequence analysis of a zein genomic clone with a short open reading frame. Gene 28: 113–118 (1984).CrossRefPubMedGoogle Scholar
  13. 13.
    Kriz A, Boston RS, Larkins BA: Structural and transcriptional analysis of DNA sequences flanking genes that encode 19 kilodalton zeins. Mol Gen Genet 207: 90–98 (1987).CrossRefPubMedGoogle Scholar
  14. 14.
    Lee KH, Jones RA, Dalby A, Tsai CY: Genetic regulation of storage protein content in maize endosperm. Biochem Genet 14: 641–650 (1976).PubMedGoogle Scholar
  15. 15.
    Maier UG, Brown JWS, Toloczyki C, Feix G: Binding of a nuclear factor to a consensus sequence in the 5′ flanking region of zein genes from maize. EMBO J 6: 17–22 (1987).Google Scholar
  16. 16.
    Marks MD, Larkins BA: Analysis of sequence microheterogeneity among zein messenger RNAs. J Biol Chem 257: 9976–9983 (1982).PubMedGoogle Scholar
  17. 17.
    Marks MD, Lindell JS, Larkins BA: Nucleotide sequence analysis of zein mRNAs from maize endosperm. J Biol Chem 260: 16451–16459 (1985).PubMedGoogle Scholar
  18. 18.
    Marks MD, Pedersen K, Wilson DR, DiFonzo N, Larkins BA: Molecular biology of the maize seed storage proteins. Curr Topics Plant Biochem Physiol 3: 9–18 (1984).Google Scholar
  19. 19.
    Maxam AM, Gilbert W: Sequencing end-labeled DNA with base-specific chemical cleavages. Meth Enzymol 65: 499–560 (1980).PubMedGoogle Scholar
  20. 20.
    Messing J, Geraghty D, Heidecker G, Hu NT, Kridl J, Rubenstein I: Genetic engineering of plant genes. In: Hollaender A, Kosuge T, Meredith CP (eds) Genetic Engineering of Plants, pp. 211–228. Plenum, New York (1983).Google Scholar
  21. 21.
    Salamini F, Soave C: Zeins: genetics and biochemistry. In: Sheridan WF (ed) Maize for Biological Research, pp. 155–160. Plant Molecular Biology Association, Charlottesville (1982).Google Scholar
  22. 22.
    Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).PubMedGoogle Scholar
  23. 23.
    Shotwell MA, Larkins BA: The biochemistry and molecular biology of seed storage proteins. In: Marcus A (ed) The Biochemistry of Plants, vol. 15, pp. 297–345. Academic Press, New York (1989).Google Scholar
  24. 24.
    Siemieniak DR, Sieu LC, Slightom JL: Strategy and methods for directly sequencing cosmid clones. Anal Biochem 192: 441–448 (1991).PubMedGoogle Scholar
  25. 25.
    Slightom JL, Sun SM, Hall TC: Complete nucleotide sequence of a French bean storage protein gene: Phaseolin. Proc Natl Acad Sci USA 80: 1897–1901 (1983).Google Scholar
  26. 26.
    Slightom JL, Drong RF, Klassy RC, Hoffman LM: Nucleotide sequences from phaseolin cDNA clones: the major storage proteins fromPhaseolus vulgaris are encoded by two unique gene families. Nucl Acids Res 13: 6483–6498 (1985).PubMedGoogle Scholar
  27. 27.
    Spena A, Viotti A, Pirrotta V: A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J 1: 1589–1594 (1982).Google Scholar
  28. 28.
    Spena A, Viotti A, Pirrotta V: Two adjacent genomic zein sequences: Structure, organization and tissue-specific restriction pattern. J Mol Biol 169: 779–811 (1983).Google Scholar
  29. 29.
    Thompson GA, Larkins BA: Structural elements regulating zein gene expression. BioEssays 10: 108–113 (1989).PubMedGoogle Scholar
  30. 30.
    Wandelt C, Feix G: Sequence of a 21 kd zein gene from maize containing an in-frame stop codon. Nucl Acids Res 17: 2354 (1989).PubMedGoogle Scholar
  31. 31.
    Wilson DR, Larkins BA: Zein gene organization in maize and related grasses. J Mol Evol 29: 330–340 (1984).Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Gary A. Thompson
    • 1
  • David R. Siemieniak
    • 2
  • Leang C. Sieu
    • 2
  • Jerry L. Slightom
    • 2
  • Brian A. Larkins
    • 1
  1. 1.Department of Plant SciencesUniversity of ArizonaTucsonUSA
  2. 2.Molecular Biology ResearchThe Upjohn CompanyKalamazooUSA

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