Plant Molecular Biology

, Volume 18, Issue 4, pp 827–833

Sequence analysis of linked maize 22 kDa α-zein genes

  • Gary A. Thompson
  • David R. Siemieniak
  • Leang C. Sieu
  • Jerry L. Slightom
  • Brian A. Larkins
Update Section Short Communication

Abstract

We have determined the nucleotide sequence of a 7343 bp zein genomic clone (gZ22.8H3) from the maize inbred W64A. Computer-aided analysis of the DNA sequence revealed two contiguous 22 kDa α-zein genes. The 5′ gene (gZ22.8) encodes a complete polypeptide and contains putative regulatory sequences in both the 5′ and 3′ flanking regions that are typical of zein genes. In contrast, the 3′ gene (ψgZ22.8) appears to be a pseudogene, because it contains numerous insertions and deletions that would prevent translation of the mRNA. Alignment of the 5′ and 3′ flanking sequences of both genes indicated that they resulted from a 3.3 kb DNA duplication event.

Key words

α-zein gene duplication maize genes maize pseudogene 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Gary A. Thompson
    • 1
  • David R. Siemieniak
    • 2
  • Leang C. Sieu
    • 2
  • Jerry L. Slightom
    • 2
  • Brian A. Larkins
    • 1
  1. 1.Department of Plant SciencesUniversity of ArizonaTucsonUSA
  2. 2.Molecular Biology ResearchThe Upjohn CompanyKalamazooUSA

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