Plant Molecular Biology

, Volume 18, Issue 4, pp 703–712

DNA methylation inhibits propagation of tomato golden mosaic virus DNA in transfected protoplasts

  • Clare L. Brough
  • William E. Gardiner
  • Nilufar M. Inamdar
  • Xian-Yang Zhang
  • Melanie Ehrlich
  • David M. Bisaro
Research Article

Abstract

The effects of methylation on plant viral DNA replication have been studied inNicotiana tabacum protoplasts transfected with DNA of the geminivirus tomato golden mosaic virus (TGMV). The transfected cells were also used to determine whether experimentally introduced methylation patterns are maintained in extrachromosomal viral DNA. Replacement of cytosine residues with 5-methylcytosine (m5C) reduced the amount of viral DNA which accumulated in transfected protoplasts. The reduction was observed whether m5C residues were substituted for cytosine residuesin vitro in either the viral strand or the complementary strand of double-stranded circular inoculum DNAs containing tandemly repeated copies of the A component of the TGMV genome. Both limited and extensive cytosine methylation of TGMV DNA sequencesin vitro was not propagated in progeny viral DNA. The absence of detectable maintenance-type methylation of the transfecting TGMV DNA sequences may be related to the lack of methylation observed in double-stranded TGMV DNA isolated from infected plants.

Key words

DNA methylation geminivirus protoplasts tomato golden mosaic virus transfection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Clare L. Brough
    • 1
  • William E. Gardiner
    • 1
    • 4
  • Nilufar M. Inamdar
    • 2
  • Xian-Yang Zhang
    • 2
  • Melanie Ehrlich
    • 2
  • David M. Bisaro
    • 1
  1. 1.Biotechnology Center and Department of Molecular GeneticsThe Ohio State UniversityColumbusUSA
  2. 2.Department of BiochemistryTulane University Medical CenterNew OrleansUSA
  3. 3.Department of Biological SciencesUniversity of DurhamDurhamUK
  4. 4.Department of Biological SciencesMississippi State UniversityStarkvilleUSA

Personalised recommendations