Plant Molecular Biology

, Volume 30, Issue 6, pp 1321–1329

Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome

  • Anu Suoniemi
  • Kesara Anamthawat-Jónsson
  • Tiina Arna
  • Alan H. Schulman
Short Communication

Abstract

The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 × 104. We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the whole of all chromosomes, excepting paracentromeric regions, telomeres, and nucleolar organizer (NOR) regions. The integrase probe showed a similar pattern. The 5′-untranslated leader (UTL) probe, expected to be the most rapidly evolving component, labeled chromosomes in a dispersed and non-uniform manner, concentrated in the distal regions, possibly indicating a targe site preference.

Key words

BARE-1 retrotransposon barley chromosomes genome organization Hordeum vulgare in situ hybridization 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Anu Suoniemi
    • 1
  • Kesara Anamthawat-Jónsson
    • 2
  • Tiina Arna
    • 1
  • Alan H. Schulman
    • 1
  1. 1.Institute of BiotechnologyUniversity of Helsinki, Biocenter 1AHelsinkiFinland
  2. 2.Agricultural Research Institute (RALA)ReykjavíkIceland

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