Plant Molecular Biology

, Volume 30, Issue 6, pp 1129–1137 | Cite as

Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata)

  • Jose R. Botella
  • Jeannette M. Arteca
  • Maria Somodevilla
  • Richard N. Arteca
Research Article

Abstract

Protein kinases are important in eukaryotic signal transduction pathways. In this study we designed degenerate oligonucleotides corresponding to two conserved regions of protein kinases and using the polymerase chain reaction (PCR) have amplified a 141 bp fragment of DNA from mungbeans (Vigna radiata Rwilcz cv. Berken). Sequence analysis of the PCR products indicates that they encode several putative protein kinases with respect to their identity with other known plant protein kinases. Using one of the six fragments (CPK3-8), we isolated a 2022 bp cDNA (VrCDPK-1) from a Vigna radiata λgt11 library. VrCDPK-1 has a 96 bp 5′-untranslated region and a 465 bp 3′-untranslated region and an open reading frame of 1461 bp. VrCDPK-1 contains all of the conserved regions commonly found in calcium dependent protein kinases (CDPK). VrCDPK-1 shares 24 to 89% sequence identity with previously reported sequences for plant CDPKs at the protein level. southern analysis revealed the presence of several copies of the CDPK gene. VrCDPK-1 expression was stimulated when mungbean cuttings were treated with CaCl2, while treatment with MgCl2 had no effect. We are reporting for the first time a CDPK gene in mungbean which is inducible by mechanical strain. Cuttings treated with indole-3-acetic acid (IAA) or subjected to salt stress showed an increase in VrCDPK-1 expression. There was a dramatic stimulation in VrCDPK-1 expression 6 h after cuttings were treated with cycloheximide.

Key words

calcium cycloheximide indole-3-acetic acid (IAA) mechanical strain protein kinase salt stress touch Vigna radiata 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Jose R. Botella
    • 1
  • Jeannette M. Arteca
    • 1
  • Maria Somodevilla
    • 1
  • Richard N. Arteca
    • 1
  1. 1.Department of HorticultureThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of BotanyThe University of QueenslandBrisbaneAustralia

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